Agricultural and biomedical application of Silver Nanoparticles synthesized by Halimeda gracilis Harvey ex J. Agardh

Agricultural and biomedical application of Silver Nanoparticles synthesized by Halimeda gracilis Harvey ex J. Agardh ( Vol-3,Issue-5,September - October 2018 )

Author: Suparna Roy, P. Anantharaman

ijeab doi crossref DOI: 10.22161/ijeab/3.5.22

Keyword: Green synthesis, seaweed, Silver nanoparticles, seed germination, antibacterial activity.

Abstract: The present work is focused on the biosynthesis of the Silver (Ag) Nanoparticles using an aqueous extract of the green seaweed Halimeda gracilis. The visible colour change indicated the biosynthesis of Silver Nanoparticles and the specific peak produced within the UV-Vis spectrum confirmed the biosynthesis of Silver Nanoparticles. The possible functional groups were identified with Fourier Infrared Spectroscopy. The morphological characterization of biosynthesized Silver Nanoparticles was done by Scanning Electron Microscopy and Dynamic light scattering measurements and Zeta potential. The average size distribution of Ag-Nanoparticles were 295.9 (d.nm) and fairly stable with a zeta potential value of -28.6 mV. The size of biosynthesized Ag-Nanoparticles was also measured with X-ray diffraction assay. Due to agglomeration, the size difference of biosynthesized Ag-Nanoparticles in case of SEM and EDX occurred. The biosynthesized Ag-Nanoparticles were assayed for their antibacterial activity against some human pathogens and for their potential on seed germination of Abelmoschus esculentus and Raphanus sativus var. longipinnatus. The antibacterial activity of biosynthesized Ag Nanoparticles was the highest against Proteus mirabilis (2.33±0.2 cm), followed by Klebsiella pneumoniae, (1±0.0 cm). The effect of biosynthesized Ag-Nanoparticles on the seed germination of Raphanus sativus var. longipinnatus was excellent as the germination rate was 100 percent for Ag-Nanoparticles treated seeds, which was better than normal seaweed extract and seaweed liquid fertilizer treated seeds. The seed germination was also good for Abelmoschus esculentus with the treatment of seaweed mediated Ag-Nanoparticles as germination rate was 60 percent. This work proved that seaweed synthesized Ag-Nanoparticles are Phyto-friendly in nature and in future nano-bio fertilizer may be used as the growth promoter and eco-friendly Nano-bio-fertilizer.

References:

[1] Bhattacharya, S., (2006). ESBL-from Petri dish to the patient. Indian. J. Med. Microbiol., 24: 20-24
[2] Gandhi, H., and Khan, S., (2016). Biological Synthesis of Silver Nanoparticles and Its Antibacterial Activity. J Nanomed Nanotechnol , 7:2
[3] Gold, H. S. (2001). Vancomycin resistant, Enterococci: Mechanism and Clinical observations. Clin. Infect. Dis. 33: 210-219
[4] Kumar, P., Selvi, S. S., Govindaraju, M., (2013). Seaweed-mediated biosynthesis of Silver Nanoparticles using Gracilaria corticata for its antifungal activity against Candida spp. Appl Nanosci - 3: 495–500
[5] Pérez, M. J., Falqué, E., and Domínguez, H., (2016). Antimicrobial Action of Compounds from Marine Seaweed, Mar. Drugs, 14, 52
[6] Barrena, R. E. Casals, J. Colon, X. Font, A. Sanchez and V. Puntes, (2009). Evaluation of the Ecotoxicity of model Nanoparticles. Chemosphere, 75 (7): 850-857.
[7] Roy, S., and Anantharaman, P. (2017). Green Synthesis of Silver Nanoparticles by Sargassum Cinctum J. Agardh and their Potential for Seed Germination , International Journal of Trend in Scientific Research and Development (IJTSRD), ISSN No: 2456 - 6470 , Volume - 1 , Issue–5
[8] Roy S, Anantharaman P (2018). Biosynthesis of Silver Nanoparticles by Sargassum Ilicifolium (Turner) C. Agardh with their antimicrobial activity and potential for seed germination. Journal of Applied Physics & Nanotechnology 1: 1-9.
[9] Sivasangari Ramya, S., Vijayanand, N., Rathinavel, S., (2015). Foliar application of liquid bio-fertilizer of brown alga Stochiospermum marginatum on growth, biochemical and yield of Solanum melongena. Int. J. Recycl Org. Waste Agricult. 4: 167–173
[10] Taga, M. S. Miller, E. E. D. E. and Pratt, (1984). Chia seeds as a source of natural lipids antioxidants. J Am Oil Chem Soc. 61(5): 928-993.
[11] Thakkar, K. N. Mhatre, S. S and Parikh, R. Y. (2010). Biological synthesis of metallic nanoparticles. Nanomedicine, 6(2): 257–262.
[12] Vivek, M., Kumar, P. S., Steffi, S., and Sudha, S., (2011). Biogenic Silver Nanoparticles by Gelidiella acerosa Extract and their Antifungal Effects, Avicenna J Med Biotechnol. 3(3): 143–148.
[13] Roy, S. and Anantharaman, P. (2018). Biosynthesis of Silver Nanoparticles by Amphiroa anceps (Lamarck) Decaisne and Its Biomedical and Ecological Implications. Journal of Nanomedicine and Nanotechnology, 9(2), pp: 2-5.
[14] Roy, S. and Anantharaman, P. (2017). Biosynthesis of Silver Nanoparticles by Chaetomorpha antennina (Bory de Saint-Vincent) Kutzing with its antibacterial activity and ecological implication. Journal of Nanomedicine and Nanotechnology, 8(5), pp: 2-9.
[15] Roy, S. and Anantharaman, P. (2018). Biosynthesis of Silver Nanoparticles by Chlorodesmis Hildebrandtii A. Gepp & E. Gepp Including its Agricultural and Biomedical Implications Nanomed Nanotechnol Biosynthesis of Silver Nanoparticles by Chlorodesmis Hildebrandtii A. Gepp & E. Gepp Including its Agricultural and Biomedical Implications. Nanomedicine & Nanotechnology, 3(3), pp: 1-9.
[16] Ganesapandian, S. Manikandan, S. Singh, M. and Kumaraguru, A. K. (2011). Emerging of multidrug resistance human pathogens from Urinary tract infections. Current Research in Bacteriology, 4 (1): 9-15.
[17] Ismail, Y. Wedyan, M. Al-Zuabe, M. and Abdurrahman, S., (2016). Antimicrobial activity of Rubia cordifolia: methods to determine antimicrobial activity. Research journal of Medicinal Plants: 10 (8): 457-462.

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Cite this Article:

MLA

Suparna Roy, P. Anantharaman et al."Agricultural and biomedical application of Silver Nanoparticles synthesized by Halimeda gracilis Harvey ex J. Agardh ". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878),vol 3, no. 5, 2018, pp.1739-1747 AI Publications doi:10.22161/ijeab/3.5.22

APA

Suparna Roy, P. Anantharaman, P.(2018).Agricultural and biomedical application of Silver Nanoparticles synthesized by Halimeda gracilis Harvey ex J. Agardh . International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).3(5), 1739-1747.10.22161/ijeab/3.5.22

Chicago

Suparna Roy, P. Anantharaman, P.(2018).Agricultural and biomedical application of Silver Nanoparticles synthesized by Halimeda gracilis Harvey ex J. Agardh . International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).3(5), pp.1739-1747.

Harvard

Suparna Roy, P. Anantharaman. 2018."Agricultural and biomedical application of Silver Nanoparticles synthesized by Halimeda gracilis Harvey ex J. Agardh ". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).3(5):1739-1747.Doi:10.22161/ijeab/3.5.22

IEEE

Suparna Roy, P. Anantharaman."Agricultural and biomedical application of Silver Nanoparticles synthesized by Halimeda gracilis Harvey ex J. Agardh ", International Journal of Environment Agriculture and Biotechnology,vol.3,no. 5, pp.1739-1747,2018.

Bibtex

@article { suparnaroy2018agricultural,
title={Agricultural and biomedical application of Silver Nanoparticles synthesized by Halimeda gracilis Harvey ex J. Agardh },
author={Suparna Roy, P. Anantharaman , R},
journal={International Journal of Environment Agriculture and Biotechnology},
volume={3},
year= {2018} ,
}