Evaluating the In vivo Efficacy of Copper-Chitosan Nanocomposition for Treating Vascular Wilt Disease in Date Palm

Evaluating the In vivo Efficacy of Copper-Chitosan Nanocomposition for Treating Vascular Wilt Disease in Date Palm ( Vol-3,Issue-2,March - April 2018 )

Author: Elwy A. Mohamed, Mohamed H. Gaber, Sherif F. Elsharabasy

ijeab doi crossref DOI: 10.22161/ijeab/3.2.17

Keyword: Copper nanoparticles, Chitosan nanoparticles, Date palm, Fusarium oxysporum, Vascular wilt.

Abstract: Date palm, Phoenix dactylifera, as one of the most important fruit crops in Egypt and many other countries, can be affected by many fungal diseases, among which the vascular wilt disease, caused by the fungal pathogen Fusarium oxysporum, is considered the most deteriorating one. This study aims at evaluating the efficiency of Copper-Chitosan Nanopcomposition for treating the vascular wilt disease in date palm. The study relies mainly on beleaguering the disease via the double-role functionality of copper-chitosan nanocomposition, i.e. its potential antifungal effect on the fungal pathogen, besides its capability to enhance the immune responses of the infected plant. In this regard, chitosan nanoparticles were prepared according to the ionic gelation method, whereas copper nanoparticles were prepared according to the chemical reduction method. Physicochemical characterization of both chitosan and copper nanoparticles was performed using dynamic light scattering (DLS), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR) and x-ray diffraction (XRD). Copper-chitosan nanocomposition could significantly reduce the vascular wilt disease severity; this means that the nanocomposition can be used in the future for developing new nano-fungicides to control such pathogens.

References:

[1] D. Karthik and K. Geetha, (2013). Synthesis of copper precursor, copper and its oxide nanoparticles by green chemical reduction method and its antimicrobial activity, Journal of Applied Pharmaceutical Science, vol. 3, no. 5, pp. 16–21.
[2] M. Muthukrishnan, (2015). Green synthesis of copper-chitosan nanoparticles and study of its antibacterial activity, Journal of Nanomedicine & Nanotechnology, vol. 6, no. 1.
[3] Agrios, G.N. (2005). Plant Pathology, Elsevier Academic Press, San Diego, California, USA.
[4] Al-Shahib W. and R. J. Marshall. (2003). the fruit of the date palm: its possible use as the best food for the future. International Journal of Food Sciences and Nutrition, 54 (4): 247–259.
[5] Anitha, A., Rani, V. D., Krishna, R., Sreeja, V., Selvamurugan, N., Nair, S. V., ... & Jayakumar, R. (2009). Synthesis, characterization, cytotoxicity and antibacterial studies of chitosan, O-carboxymethyl and N, O-carboxymethyl chitosan nanoparticles. Carbohydrate Polymers, 78(4), 672-677.‏
[6] Campbell, C.L. and Madden, L.V. (1990). Introduction to Plant Disease Epidemiology, John-Wiley & Sons, New York, NY, USA.
[7] Chandra S.; Chakraborty, N.; Dasgupta, A.; Sarkar, J. Panda, K. and Acharya, K. (2015). Chitosan nanoparticles: A positive modulator of innate immune responses in plants. Sci. Rep. 5, 15195; doi: 10.1038/srep15195 .
[8] Chester, K. S.; Horsfall, J. G. and Diamond, A. E. (1959). Plant Pathology: An AdvancesTraits, 1, Academic Press, New York, NY, pp. 199–242.
[9] Eva Petrikkou, Juan L. Rodrı́guez-Tudela, Manuel Cuenca-Estrella, Alicia Gómez, Ana Molleja, and Emilia Mellado. (2001). Inoculum Standardization for Antifungal Susceptibility Testing of Filamentous Fungi Pathogenic for Humans. J. Clin. Microbiol. vol. 39 no. 4 1345-1347.
[10] F.A.O (2014). Dates Statistics 2014. Food and Agriculture Organization of the United Nations.
[11] Flood J. (2006). A review of Fusarium wilt of oil palm caused by Fusarium oxysporum f. sp. Elaeidis. Phytopathology, 96:660-662.
[12] H. M. Yadav, S. V. Otari, V. B. Koli (2014). Preparation and characterization of copper-doped anatase TiO2 nanoparticles with visible light photocatalytic antibacterial activity, Journal of Photochemistry and Photobiology A: Chemistry, vol. 280, pp. 32–38.
[13] H. Tian, X. L. Zhang, J. Scott, C. Ng, and R. Amal, (2014). TiO2- supported copper nanoparticles prepared via ion exchange for photocatalytic hydrogen production. Journal of Materials Chemistry A, vol. 2, no. 18, pp. 6432–6438.
[14] Hajipour, M. J.; Fromm, K. M. and Akbar Ashkarran A. (2012). Antibacterial properties of nanoparticles. Trends in Biotechnology. 30: 499–511.
[15] Harish Prashanth KV and tharanathan RN (2007). Chitin/chitosan: modification and their unlimited application potential – an overview. Trends Food Sci. Techno. 18: 117-131.
[16] Hide G. A., P. J. Read and S. M. Hall (1992). Resistance to thiabendazole in Fusarium species isolated from potato tubers affected by dry rot. Plant Pathology, 41, 745–748.
[17] Ishaaya, I. (1971). Observations on the phenoloxidase system in the armored scales Aonidiella aurantii and Chrysomphalus aonidum. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 39(4), 935-943.‏
[18] Kâhkônen , M.P.; Hopia, A.I.; Vuorela, H. J.; Rauha, J. P.; Pihlaja, K.; Kujala, T. S. and Heinonen, M. (1999). Antioxidant activity of plant extracts containing phenolic compounds. J.Agric,food chem.,47 :3954-3962.
[19] Ling Yien Ing, NoraziahMohamad Zin, Atif Sarwar, and Haliza Katas (2012). Antifungal Activity of Chitosan Nanoparticles and Correlation with Their Physical Properties. International Journal of Biomaterials, doi:10.1155/2012/632698
[20] M. S. M. Suan, M. R. Johan, N. L. Hawari, and H. A. Ching, (2011). Annealing effects on the properties of copper oxide thin films prepared by chemical deposition. International Journal of Electrochemical Science, vol. 6, no. 12, pp. 6094–6104.
[21] M. Sahu and P. Biswas. (2011). Single-step processing of copper-doped titania nanomaterials in a flame aerosol reactor. Nanoscale Research Letters, vol. 6, no. 1, article 441, pp. 1–14.
[22] McDonald J.H. (2008). Handbook of Biological Statistics Sparky House Publishing, Baltimore.
[23] Mustafa B. and Ilkay S. (2010). Controlled synthesis of copper nano/microstructures using ascorbic acid in aqueous CTAB solution. Powder Technology, 198, 279–284.
[24] Patel N., P. Desai, N. Patel, A. Jha and H. K. Gautam (2014). Agronanotechnology for plant fungal disease management: a review. Int J Curr Microbiol App Sci, 3:71–84.
[25] Pelgrift, R. Y. and Friedman, A. J. (2013). Nanotechnology as a therapeutic tool to combat microbial resistance. Advanced Drug Delivery Reviews. 65: 1803–1815.
[26] Prachi. K; Sonal, B.; Swapnil, G.; Aniket, G.; Amedea, B.; Seabrab, O.; Rubilarc, D.; Nelson.; Durane, F. and Mahendra R. (2013). In vitro antifungal efficacy of copper nanoparticles against selected crop pathogenic fungi. Materials Letters, 115, 13-17.
[27] Qi, L., Xu, Z., Jiang, X., Hu, C. and Zou, X. (2004). Preparation and antibacterial activity of chitosan nanoparticles. Carbohydr. Res. 339, 2693–2700.
[28] R. Betancourt-Galindo, P. Y. Reyes-Rodriguez, B. A. Puente Urbina et al., (2014). Synthesis of copper nanoparticles by thermal decomposition and their antimicrobial properties. Journal of Nanomaterials, vol. 2014, Article ID 980545, 5 pages.
[29] Riker, A.J. and Riker, R.S. (1936). Introduction to research on plant diseases. John’s Swift Co., St. Louis, Chicago, New York and Indianapolis, 117 p.
[30] S. Magdassi, M. Grouchko, and A. Kamyshny, (2010). Copper nanoparticles for printed electronics: routes towards achieving oxidation stability. Materials, vol. 3, no. 9, pp. 4626–4638.
[31] S. T. H. Sherazi, R. A. Soomro, S. Uddin, and N. Memon, (2013). Synthesis and characterizations of highly efficient copper nanoparticles and their use in ultra fast catalytic degradation of organic dyes. Advanced Materials Research, vol. 829, pp. 93–99.
[32] Safiuddin Ansari1; Sheila Shahab; Mohd. Mazid and Dania Ahmed. (2012). Comparative study of Fusarium oxysporum f sp. lycopersici and Meloidogyne incognita race-2 on plant growth parameters of tomato. Agricultural Sciences 3: 844-847.
[33] Singleton, V. L. and Rossi, J. A. (I965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am.J.Enol.Vitic.,16:144-158.
[34] Vetter, J. L., Steinberg, M. P., & Nelson, A. I. (1958). Enzyme assay, quantitative determination of peroxidase in sweet corn. Journal of Agricultural and Food Chemistry, 6(1), 39-41.‏
[35] Wheeler, B.E.J. (1969). An Introduction to Plant Disease, John Wiley and Sons Limited,London. pp 301.
[36] X. Zhu, B. Wang, F. Shi, and J. Nie, (2012). Direct, rapid, facile photochemical method for preparing copper nanoparticles and copper patterns. Langmuir, vol. 28, no. 40, pp. 14461–14469.
[37] Xu, Yongmei and Du, Yumin (2003). Effect of molecular structure of chitosan on protein delivery properties of chitosan nanoparticles. International journal of pharmaceutics, Volume 250, Issue 1, 2 January 2003, Pages 215-226.

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Cite this Article:

MLA

Elwy A. Mohamed, Mohamed H. Gaber, Sherif F. Elsharabasy et al."Evaluating the In vivo Efficacy of Copper-Chitosan Nanocomposition for Treating Vascular Wilt Disease in Date Palm". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878),vol 3, no. 2, 2018, pp.447-454 AI Publications doi:10.22161/ijeab/3.2.17

APA

Elwy A. Mohamed, Mohamed H. Gaber, Sherif F. Elsharabasy, P.(2018).Evaluating the In vivo Efficacy of Copper-Chitosan Nanocomposition for Treating Vascular Wilt Disease in Date Palm. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).3(2), 447-454.10.22161/ijeab/3.2.17

Chicago

Elwy A. Mohamed, Mohamed H. Gaber, Sherif F. Elsharabasy, P.(2018).Evaluating the In vivo Efficacy of Copper-Chitosan Nanocomposition for Treating Vascular Wilt Disease in Date Palm. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).3(2), pp.447-454.

Harvard

Elwy A. Mohamed, Mohamed H. Gaber, Sherif F. Elsharabasy. 2018."Evaluating the In vivo Efficacy of Copper-Chitosan Nanocomposition for Treating Vascular Wilt Disease in Date Palm". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).3(2):447-454.Doi:10.22161/ijeab/3.2.17

IEEE

Elwy A. Mohamed, Mohamed H. Gaber, Sherif F. Elsharabasy."Evaluating the In vivo Efficacy of Copper-Chitosan Nanocomposition for Treating Vascular Wilt Disease in Date Palm", International Journal of Environment Agriculture and Biotechnology,vol.3,no. 2, pp.447-454,2018.

Bibtex

@article { elwya.mohamed2018evaluating,
title={Evaluating the In vivo Efficacy of Copper-Chitosan Nanocomposition for Treating Vascular Wilt Disease in Date Palm},
author={Elwy A. Mohamed, Mohamed H. Gaber, Sherif F. Elsharabasy , R},
journal={International Journal of Environment Agriculture and Biotechnology},
volume={3},
year= {2018} ,
}