Fungicidal effect of three plants extracts in control of four phytopathogenic fungi of tomato (Lycopersicum esculentum L.) fruit rot.

Fungicidal effect of three plants extracts in control of four phytopathogenic fungi of tomato (Lycopersicum esculentum L.) fruit rot. ( Vol-3,Issue-1,January - February 2018 )

Author: P. M. Ngegba, S. M. Kanneh, M. S. Bayon, E. J. Ndoko, P. D. Musa

ijeab doi crossref DOI: 10.22161/ijeab/3.1.14

Keyword: Aqueous extracts, Fungicidal effect, Inhibitory effects, Phytopathogenic.

Abstract: Fungicidal effect of leaf aqueous extracts of Azadirachta indica, Tithonia diversifolia and Chromolaena odorata were determined on rot causing fungi. In the study, the phytopathogenic fungi isolated from the infected tomato fruit parts and identified based on morphological and cultural characters were: Aspergillus niger Van Tiegh, Fusarium oxysporum Schlecht, Geotrichum candidium Link and Rhizopus stolonifer Ehrenb. ex. Fr. as confirmed by pathogenicity tests. Leaf aqueous extracts of different concentrations (20, 40, 80, 60 and 100 % w/v) of A. indica, T. diversifolia and C. odorata were added to growth media prior to inoculation. All aqueous extracts of the tested plants significantly (p < 0.05) reduced mycelial growth of the fungal pathogens and this effect gradually increased with increasing concentration. Fungicidal activity was strongly exhibited by A. indica extract at 100% w/v against all the pathogenic fungi. In the case of T. diversifolia extracts inhibitiory effects at 20, 40, 60, 80 and 100% w/v were greater than those of C. odorata on A. niger , F. oxysporum and G. candidium while for R. stolonifer inhibition, C. odorata produced the highest in the all five concentrations than T. diversifolia extracts. It could be emphatically concluded that the tested plant extracts can effectively control rot causing fungi disease of tomato. This makes them potential biocide in diseases management in that they are cheap and environmentally safe as they showed fungicidal and fungitoxic ability.


[1] Abad, Z. G., Abad, J. A. (1997). Another look at the origin of late blight of potatoes, tomatoes and pear melon in the Andes of South America. Plant Disease 81, 6: 682-688
[2] Agrios, G. N. (2005). Plant pathology. Academic Press, New York . 922pp
[3] Alexopoulos, C. J., Mims, C. W. and Blackwall, M. (2002). Introduction Mycology (4th Edition) John Wiley and Sons Inc. Singapore, 869 pp.
[4] Amadioha, A. C. and Obi, V. I. (1999). Control of anthracnose disease of cowpea by Cymbopogon citratus and Ocimum gratissimum. Acta Phytopathology Entomology Hungerica 34(2): 85-89.
[5] Asawalam, E. F. (2006). Insecticidal and repellent properties of Piper guineensis seed oil extract for the control of maize weevil, Sitophilus zeamaise. Journal of Environmental Agriculture and Food Chemistry 5:1389-1394
[6] Barnett, H. L. and Hunter B. B. (1999). Illustrated Genera of Imperfect Fungi. Fourth edition, Academic Press. San Diego California, United States of America. pp 218.
[7] Bdliya, B.S. and Aikali, G. (2008). Efficacy of some plant extracts in the management of Cercospora leaf spot of groundnut in the Sudan Savanna of Nigeria. Journal of Phytopathology Plant protection 32 (2): 154-163.
[8] Chiejina, N. V. (2008). Mycoflora of some salad vegetables. Bio-Research, 6(2): 392-395.
[9] Chiejina, N. V. and Ukeh, J. A. (2013). Efficacy of Aframomum melegueta and Zingiber officinale extracts on fungal pathogens of tomato fruits. Journal of Pharmacy and Biological Sciences 4(6):13-16
[10] Conventry, E. and Allen, E. J. (2001). Microbiological and chemical analysis of neem (Azadirachta indica) extracts: New data on antimicrobial activity. Phytoparasitica 29:441-450.
[11] Cowan, M. M. (1991). Plant products as antimicrobial agents. Clinical Microbiology Reviews 12:564-582.
[12] Das, k., Tiwari, R. K. S and Shrivastava, D. K. (2010). Techniques for evaluation of medicinal plants products as antimicrobial agents: Current methods and future trends Journal of Medicinal Plant Research 4:104-111.
[13] Ellis, D., Davis, S., Alexious, H., Handke, R. and Bartley, R. (2007) Descriptions of Medical Fungi (2nd ed). Mycology Unit Womens Hospital North Adelaid, Australia. 198 pp.
[14] Godia, A. K. G., (2014). Evaluation of some introduced fresh market tomato (Solanum lycopersicum L.) for genetic variability and adaptability in Ghana using morphological and molecular markers. M.Sc. Thesis, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
[15] Halama, P. and Van Haluwin C. (2004). Antifungal activity of lichen extracts and lichenic acids. Biocontrol 49:95-107.
[16] Hasan, M. M., Chowdhury, S. P. Alam, S. Hossain, B.and Alam, M. S. (2005). Antifungal effect of plant extracts on seed borne fungi of wheat seeds regarding seed germination, seedling health and vigour index. Pakistan Journal Biological Science 8(9):1284-1289.
[17] Ilondu, E. M. (2012). Fungitoxic activity of leaf extracts from four Asteraceae against Sclerotium rolfsii Sacc, an isolate of sweet potato ( Ipomoea batatas L.) Lam) vine rot disease. Journal of Agricultural and Biological Sciences 3(2): 287-295.
[18] Kanneh, S. M., Osei, M. K., Akromah, R. and Gyau, J. (2016). Generation mean analysis of yield and yield components of early generations of interspecific crosses of tomato (Solanum lycopersicum L.) International Journal Plant Breeding and Genetics. 10: 98-103.
[19] Koch, E., Meier, B. M., Eiben, H. G., Slusarenko, A. (1992). A Lipoxygenase from leaves of Tomato (Lycopersicion esculentum Mill.) is induced in response to plant pathogentic pseudomonds. Plant Physiology 99: 571-576.
[20] Kumar, A., Shukla, R., Singh, P., Prasad, C. S. and Dubey, N. K. (2008). Assessment of Thymus vulgaris L. essential oil as a safe botanical preservative against post-harvest fungi infestation of food commodities. Innovative Food Science and Emergence Technology, 9: 575-580
[21] Lukyanenko, A.N. (1991). Disease resistance in Genetic Improvement of Tomato. In Kallo, G. (Ed), Vol. 14 of Monographs on Theoretical and Applied Genetics, Springers, Berlyn, Germany: 99-119
[22] Moriones, E., Navas-Castillo, J. (2000). Tomato yellow leaf curl virus, an emerging virus complex causing epidemics worldwide. Virus Research 71: 123-134
[23] Ogbebor, O. N, and Adekunle, A. T. (2005). Inhibition of conidial germination of mycelial growth of Corynespora assiicola (Berk and Curt) of rubber (Hevea brasiliensis Muell. Arg.) using extracts of some plants. African Journal of Biotechnology. 4(9):996-1000
[24] Okigbo, R. N and Ogbonnaya, U, O. (2006). Antifungal effects of two tropical plant leaf extracts (Ocimum gratissimum and Aframomum molegaeta) on post-harvest yam (Dioscorea spp) rot. African Journal of Biotechnology 5(9):727-731
[25] Okigbo, R. N., Eme, U. E. Asiedu, R. and Ramesh, P. (2009). Effect of crude extracts of Allium sativum Lin, Cymopogon citratus CD. Staph and terminalia catappa on root causing fungi of dioscorea species. Nigerian Journal of Botany 22(2): 359-369.
[26] Olufolaji, D. B. (1999). Control of wet rot disease of Amaranthus spp caused by Chonehora curcubitaricum with extracts of Azadirachta indica. Journal of Sustainable Agriculture and Evironment 1 (22): 183-190.
[27] Onifade, A. K. (2002). Antifungal effect of Azadirachta indica A Juss extracts on Colletotricum lindemathianum. Global Journal of Applid Sciences 6(3): 423-428
[28] Osei, M. K., Asante, M. D., Agyeman, A., Adebayo, M. A. and Adu-Dapaah, H. (2014). Sustainable Horticultural Systems (A Tool for Achieving Food Sufficiency)
[29] Owolade, B. and Osikanlu, O. K. (1999). Evaluation of some plant extracts for the control of brown blotch disease of cowpea in South-Western Nigeria. Journal of Sustainable Agriculture and Environment. 1: 198-202.
[30] Panthee, D. R. and Chen, F. (2010). Genomics of fungal disease resistance in tomato. Curr. Genomics, 11: 30-39
[31] Scheuerella, S. and Mahaffee, W. (2002). Compost tea: Principles and prospects for plant disease control. Compost science and Utilization 10(4): 313-338
[32] Sobia, N., Yamin, B. Abdul, W. Muhammad and Sadia, S. (2011). Evaluation of anticancer activity of Debregea sissalicifolia extract against estogen receptor positive call line. African Journal Biotechnology 10: 990-995.

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