In vitro mutagenesis of Cymbidium La bell “Anna Belle” by γ-rays irradiation and oligochitosan interaction

DOI: 10.22161/ijeab/2.4.12

Keyword: Cymbidium, in vitro propagation, irradiation, mutation, oligochitosan.

Abstract: The optimum media for multiplication of protocorm like bodies (PLBs) and shoot buds of Cymbidium La bell “Anna Belle” were studied in order to prepare the in vitro samples for irradiation. The values of LD50 (lethal dose of 50% samples) of PLBs, shoot buds and plantlets of tested Cymbidium after cultivation of 4 months were also determined about 35.0, 41.0 and 83.1 Gy, respectively. The addition of oligochitosan played as an very important trigger for promotion on the generation of shoot bud from PLBs after irradiation. The in vitro variations have been generated by γ-rays irradiation of PLBs with doses in range of 20 - 50 Gy. The highest mutant frequency (3.83‰) of C. La bell was found by the irradiation of PLB samples at 30 Gy. The different properties of obtained in vitro variations compared to wild types were found to be chlorophyll, short leaves, long leaves, and violet pericardium variations. The genetic relationships among generated variant lines in M1V4 and wild type were analyzed using RAPD techniques.

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Duong Hoa Xo, Le Quang Luan et al."In vitro mutagenesis of Cymbidium La bell “Anna Belle” by γ-rays irradiation and oligochitosan interaction". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878),vol 2, no. 4, 2017, pp.1540-1550 AI Publications doi:10.22161/ijeab/2.4.12

Duong Hoa Xo, Le Quang Luan, P.(2017).In vitro mutagenesis of Cymbidium La bell “Anna Belle” by γ-rays irradiation and oligochitosan interaction. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).2(4), 1540-1550.10.22161/ijeab/2.4.12

Duong Hoa Xo, Le Quang Luan, P.(2017).In vitro mutagenesis of Cymbidium La bell “Anna Belle” by γ-rays irradiation and oligochitosan interaction. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).2(4), pp.1540-1550.

Duong Hoa Xo, Le Quang Luan. 2017."In vitro mutagenesis of Cymbidium La bell “Anna Belle” by γ-rays irradiation and oligochitosan interaction". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).2(4):1540-1550.Doi:10.22161/ijeab/2.4.12

Duong Hoa Xo, Le Quang Luan."In vitro mutagenesis of Cymbidium La bell “Anna Belle” by γ-rays irradiation and oligochitosan interaction", International Journal of Environment Agriculture and Biotechnology,vol.2,no. 4, pp.1540-1550,2017.

@article { duonghoaxo2017in,
title={In vitro mutagenesis of Cymbidium La bell “Anna Belle” by γ-rays irradiation and oligochitosan interaction},
author={Duong Hoa Xo, Le Quang Luan , R},
journal={International Journal of Environment Agriculture and Biotechnology},
volume={2},
year= {2017} ,
}