In vitro regeneration and conservation of Indigo (Indigofera tinctoria L.) by slow growth induction

DOI: 10.22161/ijeab/1.4.35

Keyword: Axillary meristem culture, genetic stability, in vitro conservation, shoot proliferation, slow growth induction.

Abstract: An efficient protocol for rapid in vitro clonal propagation has been established. Shoot proliferation was obtained from nodal explants in Murashige and Skoog medium supplemented with 1.0 mg L-1BA and 0.1 mg L-1 IAA. The shoots were subsequently subcultured every four weeks. The shoots were then rooted in vitro in MS medium supplemented with varying concentrations of auxins. The most efficient rooting was observed in MS supplemented with 1.5 mg L-1 IAA. The effective acclimatization (91.67 per cent) was obtained in sterilized sand. Slow growth was induced with varying concentration of mannitol (5 g L-1 to 30 g L-1). Maximum slow growth induction with 100 per cent regeneration was recorded in mannitol (10 g L-1) supplemented medium. After 28 weeks in slow growth medium, cultures when transferred to shoot proliferation medium gave 100 per cent regeneration. The regenerants from slow growth induction were found to be genetically stable.

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Nair D.S., Reghunath B.R., Soni K.B., Alex S et al."In vitro regeneration and conservation of Indigo (Indigofera tinctoria L.) by slow growth induction". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878),vol 1, no. 4, 2016, pp.0873-0884 AI Publications doi:10.22161/ijeab/1.4.35

Nair D.S., Reghunath B.R., Soni K.B., Alex S, P.(2016).In vitro regeneration and conservation of Indigo (Indigofera tinctoria L.) by slow growth induction. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).1(4), 0873-0884.10.22161/ijeab/1.4.35

Nair D.S., Reghunath B.R., Soni K.B., Alex S, P.(2016).In vitro regeneration and conservation of Indigo (Indigofera tinctoria L.) by slow growth induction. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).1(4), pp.0873-0884.

Nair D.S., Reghunath B.R., Soni K.B., Alex S. 2016."In vitro regeneration and conservation of Indigo (Indigofera tinctoria L.) by slow growth induction". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).1(4):0873-0884.Doi:10.22161/ijeab/1.4.35

Nair D.S., Reghunath B.R., Soni K.B., Alex S."In vitro regeneration and conservation of Indigo (Indigofera tinctoria L.) by slow growth induction", International Journal of Environment Agriculture and Biotechnology,vol.1,no. 4, pp.0873-0884,2016.

@article { naird.s.2016in,
title={In vitro regeneration and conservation of Indigo (Indigofera tinctoria L.) by slow growth induction},
author={Nair D.S., Reghunath B.R., Soni K.B., Alex S , R},
journal={International Journal of Environment Agriculture and Biotechnology},
volume={1},
year= {2016} ,
}