Antimicrobial and antioxidant activities of salt stress callus of Brinjal (Solanum melongena L.)

Antimicrobial and antioxidant activities of salt stress callus of Brinjal (Solanum melongena L.) ( Vol-2,Issue-5,September - October 2017 )

Author: K. Kalimuthu, A.Vanitha, Vajjiram Chinnadurai, R. Prabakaran

ijeab doi crossref DOI: 10.22161/ijeab/2.5.23

Keyword: Antioxidant, , Inhibition, In vitro, Stress tolerant callus, Solanum melongena.

Abstract: Ethanolic and methanolic salt stress callus extracts of Solanum melongena L. were tested for in vitro antimicrobial and free radical scavenging assayssuch as DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS+(2,2’Azinobis (3-ethyl benzo-thizoline-6-sulfonic acid) . In both the extracts the zone of inhibition is higher in Escherichia coli, Klebsiella pneumonia, Staphylococcus aureusand Streptococcus pyogenesat 90 µl concentration against the control. The antifungal activity of these extracts also the zone of inhibition is higher at 90 µl concentration against the control. The DPPH activity of different concentration of solvent extracts (1 mg/ml to 5 mg/ml) along with standard ascorbic acid among the five different concentration (50 µg/ml to 250 µg/ml) of extracts tested, the higher percentage of inhibition was observed in 250 µg/ml of methanol extract followed by ethanolic extract against the standard ascorbic acid. In ABTS+ activity the absorbance was increased with the increasing concentrations of both methanolic and ethanolic callus extracts.


[1] Sathyaprabha G, Kumaravel S, Ruffina D,Praveenkumar P. A comparative study on antioxidant, proximate analysis, antimicrobial activity and phytochemical analysis of Aloe vera and Cissusquadrangularis by GC-MS. J. Pharm Res. 2010;3:2970-73.
[2] Burt S. Essential oils: their antibacterial properties and potential applications in foods, A review. Int J Food Microbiol.2004;94(3):223-53.
[3] Kim SJ, Cho AR, Han J. Antioxidant and antimicrobial activities of leafy green vegetable extracts and their applications to meat product preservation. Food Control.2013;29:112-20.
[4] Basavaraju R. Plant tissue culture-Agriculture and health of man. Indian J Sci& Tech.2011; 4(3):333-35.
[5] Khan R.Solanum melongena and its ancestral forms. Hawkes J, Lester R. Skelding A (eds.) In: The Biology and Taxonomy of the Solanaceae. 1979:629-36.
[6] Jorge PA, Neyra LC, Osaki RM, de Almeida E,Bragagnolo N. Effect of eggplant on plasma lipid levels, lipidic peroxidation and reversion of endothelial dysfunction in experimental hypercholesterolemia.Arq BrasCardiol. 1998;70:87-91.
[7] Silva ME, Santos RC, O'Leary MC,Santos RS. Effect of aubergine (Solanum melongena) on serum and hepatic cholesterol and triglycerides in rats. Braz Arch Biol Tech. 1999;42:339-42.
[8] Igarashi K, Yoshida T, Suzuki E. Antioxidative activity of nasunin in choujanasu (little eggplant, Solanum melongena L. Chouja). J JpnSoc Food Sci. 1993;40:138-43.
[9] Noda Y, Kneyuki T, Igarashi K, Mori A, Packer L. Antioxidant activity of nasunin, an anthocyanin in eggplant.Res CommunMolPatholPharmacol.1998;102:175-87.
[10] Yoshikawa K, Inagaki K, Terashita T, Shishiyama J, Kuo S,Shankel DM. Antimutagenic activity of extracts from Japanese eggplant.Mutat Res Genet Toxicol Test.1996a;371:65-71.
[11] Gafner S, Woffender JL, Nianga M,Hostettmann K. Phytochemistry.1998;48:215.
[12] Anonymous.Pharmacopiea of India (The Indian Pharmacopiea). 3rdEdn, Govt of India. New Delhi. Ministry of Health and Family Welfare. 1996.
[13] Taylor RSL, Manandhar NP, Hudson JB,Towers GHN. Screening of selected medicinal plants of Nepal for antimicrobial activities,.JEthnopharma. 1995;546:153-59.
[14] Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. JAgric Food Che. 1992;40:945-48.
[15] Giao MS, Gonzalez SML, Rivero PMD, Pereira CI, Pintado ME,Malcata FX. Infusions of Portuguese medicinal plants. Dependence of final antioxidant capacity and phenolic content on extraction features. J Sci Food Agri. 2007;87:2638-47.
[16] Neves JM, Matos C, Moutinho C, Queiroz G,Gomes LR.Ethnopharmacological notes about ancient uses of medicinal plants in Tras-os-Montes (northern of Portugal). J. Ethnopharm. 2009;124(2):270-83.
[17] Hammer KA, Carson C, Riley T. Antimicrobial activity of essential oils and other plant extracts.J AppliMicrobio.2001; 86(6):985-90.
[18] Ajila CM, Naidu KA, Bhat SG, Prasada RU, Rao JS. Bioactive compounds and antioxidant potential of mango peel extract. Food Che.2007;105:982-88.
[19] Ramachandra RS,Ravishankar GA. Plant cell cultures: chemical factories of secondary metabolites. Biotech Adv.2002;20:101–53.
[20] Koca H, Bor M,Ozdemir F. The effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content of sesame cultivars.Environ Exp Bot.2007;60:344-51.
[21] George Daye Mandy C, Waribo Helen Anthony,Okpara Kingsley. Detection of antimicrobial and antimycotic activities of African garden egg fruits (S.melongenaL.) against pathogenic organisms in solvents of varying polarities. Scholars Research Library. 2014;6(6):443-47.
[22] Rhoades JD, Loveday J. Salinity in irrigated agriculture. In American Society of Civil Engineers. Irrigation of Agricultural Crops.American Society of Agronomists.1990;2:1089-42.
[23] Mulabagal V, Lee CY, Shu-Fung L, Satish MN, Chien YL,et al,. Studies on the production of some important secondary metabolites from medicinal plants by plant tissue cultures. Bot Bull Acad Sin.2004;45:1-22.
[24] Santhi M,Muthulakshmi S, Gurulakshmi G,Rajathi S.Effect of salt stress on physiological and biochemical characteristics in Solanum nigrum L. Int J Sci& Res. 2013;6:14.
[25] Saraf P, Suva RS. Comparison of antimicrobial efficacy of ginger and eggplant leaves against clinical isolates of diabetic foot ulcers. General Practitioner.2009; 17(1): 7-9.
[26] Amutha S. Screening of antibacterial activity of Solanum melongena seed extracts on selected human pathogenic bacteria. Int J Pharm Bio Sci.2014; 5(4):208-13.
[27] Kent FM, Louise VT, Paul VA, Malendia MM, David RR, Dennis LC,et. al,. Metabolic compensation of steroidal glycoalkaloid biosynthesis in transgenic potato tubers: using reverse genetics to confirm the in vivoenzyme function of a steroidal alkaloid galactosyltransferase.Plant Sci. 2004;168(1):267-73.
[28] Rodriguez-Saona LE, Wrolstad RE, Pereira C.Glycoalkaloid content and anthocyanin stability to alkaline treatment of red fleshed potato extracts.J Food Sci.1999;64(3):445-50.
[29] Imdadul H, Wirakarnain S, Koshy P, Arash R, Shariff HABM, Mat TR. Valuable antioxidant and antimicrobial extracts from Rhizophoramucronataof Asiatic mangrove forests.Res J Biotech.2011a;6(1):10-14.
[30] Natarajan V, Venugopal P, Menon T. Effect of Azadirachta indica (Neem) on the growth pattern of dermatophytes.Indian J Med Micro bio.2003;21(2):98-101.
[31] Imdadul H, Wirakarnain S, Shariff HABM, Mat TR,Monneruzzaman KM. Total phenolic contents, antioxidant and antimicrobial activities of Bruguieragymnorrhiza.J Med Plant Res.2011b;5(17): 4112- 18.
[32] Ashraf M, Ali Q. Relative membrane permeability and activities of some antioxidant enzymes as the key determinants of salt tolerance in canola (Brassica napusL.).Environ Exp Bot. 2008;63: 266- 73.
[33] Bushra Sultana, ZaibHussain, Munazza Hameed, Muhammad Mustaq. Antioxidant activity among different parts of Aubergine (Solanum melongena L. ). Pak J Bot.2013;45(4):1443-48.
[34] SrideviMuruhan, SenthilSelvaraj, PugalendiKodukkurViswanathan. In vitro antioxidant activities of Solanum surattense leaf extract. A Pacific J TropiBiomedi.2013;3(1): 28-34.
[35] Awika JM, Roony LM, Wu X, Prior RL,Zevallos LC. Screening methods to measure antioxidant activity of Sorghum (Sorghum bicolor) and sorghum products. J Agri Food Che. 2003;51:6657-62.
[36] Galato D, Ckless K, Susin MF, Giacomelli C, Ribeiro-do-Valle RM,Spinelli A. Antioxidant capacity of phenolic and related compounds, correlation among electrochemical, visible spectroscopy methods and structure-antioxidant activity. Redox Report. 2001;6(4):243-50.
[37] Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agri Food Che.2001;49:5165-70.
[38] Anushree Tiwari, Rajesh, Jadon S, Piyush Tiwari,Nayak S. Phytochemical investigations of crown of Solanum melongena fruit.Int J Phytomedi. 2009;1:9-11.
[39] Kwon YI, Apostolidis E, Shetty K.In vitro studies of eggplant (Solanum melongena) phenolics as inhibitors of key enzymes relevant for type 2 diabetes and hypertension.Bioresour Tech.2008; 99(8):2981 - 88.
[40] Loredana Salerno, Maria N, Modica, Valeria Pittalà, Giuseppe Romeo, Maria A,Siracusa, Claudia Di Giacomo, Valeria Sorrenti, Rosaria acquaviva. Antioxidant activity and phenolic content of microwave-assisted Solanum melongena extracts. Sci World J.2014;1:1-6.
[41] Ghoson Saleh S. Chemical detection of some active compounds in eggplant (Solanum melongena) callus as compared with fruit and root contents.Int J Curr Microbio App Sci.2015;4(5):160-65.

Cite this Article: Show All (MLA | APA | Chicago | Harvard | IEEE | Bibtex)

Total View: 230 Downloads: 21 Page No: 2448-2456