Determination of Physiological, Biochemical and Molecular Effects of Zinc Stress on the Growth of Sunflower Seedlings (Helianthus annuus L.)

Determination of Physiological, Biochemical and Molecular Effects of Zinc Stress on the Growth of Sunflower Seedlings (Helianthus annuus L.) ( Vol-3,Issue-2,March - April 2018 )

Author: Ekrem Bolukbası, Aysenur Bolukbası, Sumer Aras

ijeab doi crossref DOI: 10.22161/ijeab/3.2.29

Keyword: Genotoxic effect; Heavy metal; Sunflower; Zinc stress.

Abstract: Heavy metal contamination is an important environmental problem all over the world. High concentrations of heavy metals cause permanent damage stocells and tissues. In this study, the toxic effects of zincheavy metal in sunflower plant in population and molecular parameters were investigated. The effects of zincheavymetalon sunflower seedlings were determined using the changes in population parameters; rootlength, dry weight, and total solubleprotein content. Total protein content of sunflower plants was determined in a relationship in the opposite direction increasing the amount of metal concentrations. Genotoxic effects of heavymetal of zinc on sunflower plants were revealed by using changes in genomic template stability (GTS). According to analyses, serious changes in genomic template stability were detected and these results were compared with the growth, dry weight and total soluble protein content of the seedlings grown at various zinc concentrations. Also, it was seen that the genomic template stability significantly affected the primary root length, root dry weight and root total soluble protein content. Positive correlations were observed between physiological, biochemical and molecular parameters in sunflower seedlings under zinc stress.In conclusion, a comparison between physiological, biochemical and molecular parameters shows that zinc is a genotoxic agent for sunflower plants.

References:

[1] Aksoy, D.and Aras, S. 2010.Evaluation of copperstress on eggplant (Solanummelongena L.) seedlings at molecular ve population level susing various biomarkers. MutationResearch.719: 29-34.
[2] Atienzar, F. A., Venier, P., Jha, A. N. and Depledge, M.H. 2002. Evaluation of the random amplified polymorphic DNA (RAPD) assay for the detection of DNA damage and mutations. Mutation Research. 521: 151-163.
[3] Barcelo, J. and Poschenrieder, C. 1990. Plant water relations as effected by heavy metal stres. Journal of PlantNutrition.13: 1-37.
[4] Batir, M. B., Candan, F., Buyuk, I.andAras, S.2015.Thedetermination of physiological and DNA changes in seedlings of maize (Zeamays L.) seed sex posed to the waters of the Gediz River and copper heavy metal stress. Environmental Monitoring and Assessment.187: 169-175.
[5] Batir, M. B., Candan, F.andBuyuk, I. 2015.Determination of the DNA changes in theartichokeseedlings (Cynarascolymus L.) subjected to lead and copperstresses. Plant Soiland Environment. 62(3):143-149.
[6] Bekiaroglu, P.andKarataglis, S. 2002.Theeffect of leadandzinc on Menthaspicata. Journal of AgronomyandCropScience.188: 201-205.
[7] Blackmana, B. K.,Scascitellic, M., Kanec, N. C., Lutona, H. H., Rasmussena, D. A., Byed, R. A., Lentze, D. L.andRieseberga, L. H. 2011.Sunflower domesticationalleles support single domestication center in eastern North America. Proceedings of theNational Academy of Science.108(34): 14360-14365.
[8] Bradford, M. M. 1976. A rapid and sensitive method forth equantitation of microgram quantities of protein utilizingthe principle of protein-dyebinding. Analytical Biochemistry.72: 248-254.
[9] Burzynski, M.and Klobus, G. 2004. Changes of photosynthetic parameters in cucumber leaves under Cu, Cd and Pb stress. Photosynth.42(4):505-510
[10] Cansaran,D.,Beyaztas, T., Atakol, O.and Aras, S. 2011.Assesment of the air pollution genotoxicity by RAPD in Everniaprunastri L. Ach. provinceof iron-steelfactory in Karabuk, Turkey. Journal of EnvironmentalSciences.23: 1171-1178.
[11] Hall, J. L. 2002. Cellular mechanisms for heavy metal detoxification and tolerance. Journal of Experimental Botany.53: 1-11.
[12] Kastori, R.,Petrović, M.andPetrović, N. 1992.Effect of excesslead, cadmium, copper, andzinc on waterrelations in sunflower. Journal of PlantNutrition.15: 2427-2439.
[13] Khurana, N.andChatterjee, C.2001.Influence of variablezinc on yield, oilcontent, andphysiology of sunflower. Communications in Soil Science and Plant Analysis. 32: 3023-3030.
[14] Kirbag, F.andMunzuroglu, O. 2006.Toxiceffects of cadmium (Cd2+) on metabolism of sunflower (Helianthusannuus L.) seedlings. Acta Agriculturae Scandinavica,Section B-Soil & PlantScience.3(56): 224-229.
[15] Koc, E., Ustun, A.S.andArici, Y. 2012. Effect of Different Zinc Concentrations on Total Protein, Hydrogen Peroxide Content and Peroxidase Activity in Pepper (Capsicum annuum L.) Seedlings. ArtvinCoruh University, Journal of Forestry Faculty.13(2): 205-212.
[16] Lefort, F., Lally, M., Thompson, D.and Douglas, G. 1998. Morphological traits, microsatellite fingerprinting and genetic relatedness of a stand of elite oaks (Q. robur L.) at Tullynally, Ireland, Silvae.Genetica.47: 257-261.
[17] Lentz, D. L., Pohl, M. D., Alvarado, J. L., Tarighat, S.and Bye, R. 2008. Sunflower (Helianthus annuus L.) as a pre-Columbian domesticate in Mexico. Proceedings of the National Academy of Science.105: 6232-6237.
[18] Levitt, J. (1972).Responses of plantstoenvironmentalStresses. New York, London: AcademicPress. 697.
[19] Liu, W., Li, P., Qi, X., Zhou, Q., Zheng, L., Sun, T.and Yang, Y. 2005. DNA changes in barley Hordeum vulgare seedlings induced by cadmium pollution using RAPD analysis.Chemosphere.61: 158-167.
[20] Liu, W., Yang, Y. S., Zhou, Q. X., Xie, L. J., Le, P. J.and Sun, T. H. 2007. Impact assessment of cadmium contamination on rice (Oryza sativa L.) seedlings at molecular and population levels using multiple biomarkers. Chemosphere. 67: 1155-1163.
[21] Savva, D.2000. The use of arbitrarily primed PCR (AP-PCR) fingerprinting to detect exposure to genotoxic chemicals. Ecotoxicology. 9: 341-353.
[22] Soydam, A. S.,Gokce, E., Buyuk, I.and Aras, S. 2012.Characterization of stress induced by copper and zinc on cucumber (Cucumissativus L.) seedlingsbymeans of molecular and population parameters. Mutation Research.746: 49-55.
[23] Steinkellner, H., Mun-Sik, K., Helma, C., Ecker, S., Ma, T. H., Kundi, M. and Knasmuller, S. 1998.Genotoxic effects of heavymetals: comparative investigation with plant bioassays. Environmental and Molecular Mutagenesis. 31: 183-191.
[24] Yarsan, E., Bilgili, A.andTurel, I. 2000.Heavy metal levels in mussels (UniostevenianusKrynicki) obtainedfrom Van Lake. TurkishJournal of Veterinary&AnimalSciences.24: 93-96.
[25] Zengin, F. K.andMunzuroglu, O. 2004.Effect of lead (Pb2+) andcopper (Cu2+) on thegrowth of root, shootandleaf of bean (Phaseolusvulgaris L.) seedlings. Gazi UniversityJournal of Science.173: 1-10.

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