Diversity study of Drumstick (Moringaoleifera Lam.) using Microsatellite markers

Diversity study of Drumstick (Moringaoleifera Lam.) using Microsatellite markers ( Vol-2,Issue-5,September - October 2017 )

Author: Amao A.O., Echeckwu C.A., Aba D.A., Katung M.D., Odeseye A.O.

ijeab doi crossref DOI: 10.22161/ijeab/2.5.14

Keyword: Moringaoleifera, molecular diversity, SSR Markers, gene diversity, PIC value.

Abstract: The study of the magnitude of genetic diversity existing within thirty one accessions of Moringaoleifera collections made within and outside Nigeria was conducted using ten Randomised Amplified Polymorphic DNA and tenMicrosattellite markers.None of the RAPD showed amplification bands. Five out of the Microsattellites markersamplified, four primers MO1, MO10, MO15 and MO41 were polymorphic in nature while the marker MO6 produced only a monomorphic band.PIC value was highest for the primer MO41 with 0.75 followed by primer MO1 with 0.68 while, the lowest PIC value was recorded by the primer MO15 with 0.11.A total of 19 alleles were produced by the four primers and the number of alleles ranged from two to nine with an average of 4.75 alleles per primer. The maximum number allele frequency was generated by primer MO15 followed by MO10.The gene diversity varied from 0.12 to 0.78 with an average of 0.52, PIC content of the SSR primers ranged from 0.11 to 0.75 with an average of 0.48 with primers MO 41 followed closely by primer MO1 having maximum numbers of allele number, PIC and gene diversity. Hence, the primer pairs MO41and MO1 can be considered in future molecular studies of Moringaoleifera.The Cluster analysis was able to group the thirty one accessions into two main clusters with four sub clusters. Six of the accessions were found to be duplicated or closely related with one or two other accessions having 0.00 genetic distances between them. The clusters were having some accessions grouped based on same area of collection, however there still existed groupings that were not having link with area of collection.


[1] Anwar F, Bhanger M., (2003).Analytical characterization of Moringaoleifera seed oil grown in temperate regions of Pakistan. J Ag Food Chem 51:6558–6563
[2] Brown,W.L. (1983). Genetic diversity and genetic vulnerability- An appraisal.Econ.Bot. 37(1): 4-12
[3] Elmagboul, H., Mahgoup, S., Eldoma, A. (2014). Variation in seed morphometric characteristics and germination of Acacia tortilissubspecies raddiana and subspecies spirocarpaamong three provenances in Sudan.Global Journal of Bio-Science and Biotechnology. 3(2): 191–196.
[4] FRIN (2015). Forestry Research Institute of Nigeria, Annual Meterological Report.
[5] Fugile, L. T., (2013): Moringaoleiferanatural nutrition for the tropics, Dakar world Service published as the miracle trees.
[6] Ganesan, S.K.; Singh, R.; Roy Choudhury, D.; Bharadwaj, J.; Gupta, V.; Singode, A. 2014. Genetic diversity and population structure study of drumstick (MoringaoleiferaLam.) using morphological and SSR markers. Ind. Crop. Prod. Vol. 60, 316–325.
[7] Kuo, G. (2002). Annual Report.Asian Vegetable Research Development Centre, Taiwan, pp.133-134.
[8] Kuroda, Y.; Tomooka, N.; Kaga, A.; Wanigadeva, S.M.S.W.; Vaughan, D.A. (2009).Genetic diversity of wild soybean (Glycine sojaSieb.EtZucc.) and Japanese cultivated soybeans [G.max (L.) Merr.]based on microsatellite (SSR) analysis and the selection of a core collection. Genetic Resources and CropEvolution, v.56, p.1045-1055.
[9] Mgendi, M., Manoko, M., Nyomora, A.M. (2010) A. Genetic diversity between cultivated and non-cultivated MoringaoleiferaLam. provenances assessed by RAPD markers .Journal of Cell Molecular Biology, 8, 95–102.
[10] MondiniLinda, ArshiyaNoorani and Mario A. Pagnotta (2009) Assessing plant genetic diversity by Molecular tools. Diversity Vol. 1, 19-35. www.mdpi.com/journal/diversity
[11] Morton, J.F.,(1991). The horseradish tree, Moringapterygosperma (Moringaceae)—A boon to Arid Lands?Econ.Bot.45, 318–333.
[12] Muluvi, G. M., Sprent, J. L., Soranzo, N., Provan, J., Odee, D., Folkard. G., McNicol, J. W., Powell, W. (1999). Amplified fragment length polymorphism (AFLP) analysis of genetic variation in MoringaoleiferaLam. Molecular Ecology. 8: 463-470.
[13] Muluvi, G.M.; Sprent, J.I.; Odee, D.; Powell, W. (2004). Estimates of outcrossing rates in Moringaoleiferausing Amplified fragment length polymorphism (AFLP). Afr. J. Biotechnol., 3,145–151
[14] Rajalakshmi R., Rajalakshmi S., and Parida Ajay (2017). Evaluation of the genetic diversity and population structure in drumstick (MoringaoleiferaL.) using SSR markers. Current Science 112. 6, 25. doi: 10.18520/cs/v112/i06/1250-1256
[15] Rao, V. Ramanatha and Hodgkin, Toby (2001).Genetic diversity and conservation and utilization of plant genetic resources.Plant Cell, Tissue and Organ Culture 68: 1-19
[16] Rufai, S., Hanafi, M.M., Rafii, M.Y., Ahmad, S., Arolu, I.W., Ferdous, J. (2013). Genetic Dissection of New Genotypes of Drumstick Tree (Moringaoleifera Lam.) Using Random Amplified Polymorphic DNA Marker. BioMed Research International, http://dx.doi.org/10.1155/2013/604598
[17] Saini, R.K., Saad, K.R., Ravishankar, G.A., Giridhar, P., and Shetty, N.P. (2013). Genetic diversity of ommercially grown Moringaoleifera Lam. cultivars from India by RAPD, ISSR and cytochrome P450-based markers. Plant System Evolution 299, 1205–1213.
[18] Salvakumari P., and Ponnuswami V., (2015) Genetic diversity of Moringaoleifera using SSR markers .International Journal of Tropical Agriculture. 33, No2 943-946
[19] Sanchez N, Sporndly E, Ledin I (2006) Effect of feeding different levels of foliage of Moringaoleifera to creole dairy cows on intake, digestibility, milk production and composition. Livestock Sci 101:24–31
[20] Stephenson K, Fahey J (2004) Development of tissue culture methods for the rescue and propagation of endangered Moringa spp. germplasm.Econ Bot 58:S116–S124
[21] Tanksley, S. D., Young, N. D., Paterson, A. H andBonierbale, M. W. (1989). RFLP mapping in plant breeding: Newtools for an old science. Biotechnol., 7: 257-264.
[22] Wu, J.C.; Yang, J.; Gu, Z.J.; Zhang, Y.P. 2010. Isolation and characterization of twenty polymorphic microsatellite loci for Moringaoleifera(Moringaceae).HortScienceVol.,45, 690–692.
[23] Zenglu, L., and Randall, L.N. ( 2002). RAPD marker diversity among cultivated and wild Soybean accessions from four Chinese provinces. Crop Science. 42, 1737–1744.

Cite this Article: Show All (MLA | APA | Chicago | Harvard | IEEE | Bibtex)

Total View: 67 Downloads: 7 Page No: 2380-2386