Effectiveness Trichoderma and Beauveria bassiana on Larvae of Oryctes rhinoceros On Palm Oil Plant (Elaeis Guineensis Jacq.) In Vitro

Effectiveness Trichoderma and Beauveria bassiana on Larvae of Oryctes rhinoceros On Palm Oil Plant (Elaeis Guineensis Jacq.) In Vitro ( Vol-3,Issue-1,January - February 2018 )

Author: Lita Nasution, Riahta Corah, Nuraida, Ameilia Zuliyanti Siregar

ijeab doi crossref DOI: 10.22161/ijeab/3.1.20

Keyword: Effectiveness, Trichoderma, Bauveria, Orycetes, Oil Palm Oil, Vitro.

Abstract: O. rhinoceros horn beetle (Coleoptera: Scarabaeidae) is the main pest attacking oil palm crops in Indonesia, especially in palm oil rejuvenation areas. The study was conducted from April to August 2016 in the laboratory of the Faculty of Agriculture, Al-Azhar University, Medan. The materials used in this research are horn beetle pest larvae (O. rhinoceros) originating from PT. Socfin Indonesia, Trichoderma sp fungi originating from the Food Crops and Horticultural Fields of Medan and the B. bassiana fungi are derived from the Plant Seed Plant Protection Center (PBPPTP), aqua pro injection, Tween 80, rice and 96% alcohol. This research uses Completely Randomized Design (RAL) Non Factorial consisting of 13 treatments. The result of mortality of O. rhinoceros larvae on 1-14 DAA observation can be seen in appendix 2-43. Based on fingerprint analysis showed that the application of Trichoderma sp and B. basianna fungi on O. rhinoceros larvae had no significant effect on observation of 1 DAA to 9 DAA, but had a very significant effect on observation of 10 DAA to 14 DAA. The results of germination of Trichoderma sp and B. basianna fungus 4 hours after incubation period can be seen in appendix 61-66. Precentages mortality of the highest larvae of O. rhinoceros to Trichoderma sp fungus with a dose of 20 gr (96.67%) with the application method spread on the larvae. The highest amount of conidial density is found in Trichoderma sp fungi with doses of 60 g x 100-1 ml of aqua pro injection of 7.25 x 106 conidia/ml.Germination level of conidia mushroom highest in Trichoderma sp fungi with dose 60 gr x 100-1 ml aqua pro injection that is as much as 91%.

References:

[1] Ahmad, RZ. 2008. Utilization of Fungi to Increase Productivity and Animal Health. J. Agricultural Research and Development 27: 84-92.
[2] Apriyaldi, R. 2015. Intensity Analysis of Horse Attack of Horn Beetle (Oryctes rhinoceros) In Palm Oil Plantation at PTPN V Sei Galuh Kampar Regency of Riau Province. Final Project.12-17.
[3] Bai NS, TO Sasidharan, OK Remadevi, PD Rajan, M Balachander. 2010. Virulence of Metharhizium Isolates Against The Polyphagous Defoliator Pest, Spilarctia oblique (Lepidoptera: Arctiidae). J. Trop. Forest Sci. 22 (1) : 74-80.
[4] Barron. 2011. Beauveria bassiana. (Uoguelph.ca/-g barron/Miscelineous). Retrieved 20 April 2011.
[5] Budi, AS, Afandhi, A and Puspitarini, RD. 2013. Pathogenicity of Entomopathogenic Fungus of Beauveria bassiana Balsamo (Deuteromycetes: Moniliales) On Spodoptera Larva litura Fabricius (Lepidoptera: Noctuidae). Journal of Plant Pests 1 (1): 57-65.
[6] Brotodjojo, R.R and Solichah, C. 2012. Effectivity of Entomopathogenic Fungus Beauveria bassiana to Control White Grub Lepidiota sp. 1-10.
[7] Chamzurni T, Oktarina H, and Hanum, K. 2013. Effectiveness Trichoderma harzianum and Trichoderma virens To Control Rhizoctonia solani Kuhn On Chili Seed (Capsicum annum L.). Agrista Journal 17 (1): 12-17.
[8] Clarkson Charnley. 2013. Boophilus Microplus Infection by Beauveria amorpha and Beauveria bassiana: SEM Analysis and Regulation Of Subtilisin-Like Proteases and Chitinases. Current Microbiol 5: 257-261.
[9] Dinata, AJ. 2012. Pathogenic Fungi and Bacteria. Common Wealth Agricultural Bureaux. England. 233.
[10] Effendi, TA, Septiadi, R, Salim, A, Mazid, A. 2010. Fungi Entomopatogen Origin of Lebak Land in South Sumatra and its Potential as Biological Agent of Walang Sangit (Leptocorisa oratorius (F.) Journal of Tropical Plant Disease Pest (2): 154-191 ISSN 1411-7525.
[11] Gandjar. 2012. Atlas of Entomophatogenic Fungi. Springer- Verla. New York. 187.
[12] Hanafiah, K.A. 2010. The Design of Theory and Application Trials. Jakarta. PT. Raja Grafindo Persada. 33-43.
[13] Harjaka, T, Martono, E, Witjaksono, and Sunarminto, B.H. 2015. Potential Mushroom Metarhizium anisopliae For Urethral Control of Sugar Cane Root. National Seminar of Pesnab IV. 91-98.
[14] Hashim, A, Nuraida, and Trizelia. 2009. Pathogenicity of Entomopathogenic Fungi Against Stadia Egg and Pest Larvae Cabbage Crocidolomia pavonana Fabricuius. Journal of Horticulture 12:45-49. ISSN 0853-7097.
[15] Heriyanto and Suharno. 2008. Pathogenic Studies Metarhizium anisopliae (Meth.) Sor ResultsLong Liquid Natural Medium on Oryctes rhinoceros Larvae. J. Agricultural Sciences 4 (1): 47-54.
[16] Islam, M.T and Omar, D.B. 2015. Combined Effect of Beauveria bassiana With Neem on Virulence of Insect in Case of Two Application Approaches. The Journal of Animal & Plant Sciences 22. (1): 77-82.
[17] Junaedi D, Bakti, D and Zahara, F. 2015. Predation Power Myopopone castaneae (Hymenoptera: Formicidae) Against Larvae Oryctes rhinoceros L. (Coleoptera: Scrabaidae) at the Laboratory. Journal of Agroecotechnology 3 (1): 112-117. ISSN 2337-6597.
[18] Kaur, S, Kaur, HP, Kaur K, and Kaur A. 2011. Effect of Different Concentrations of Beauveria bassiana on Development and Reproductive Potential of Spodoptera litura (Fabricius). Journal of Biopesticides 4 (2): 161-168.
[19] Khairunnisa, S, Pinem, MI, and Zarah, F. 2014. Effectiveness Test of Entomopatogen Nematodes as Controlling Borrow Oil Borrow (Oryctes rhinoceros L.) (Coleoptera: Scarabaidae) in the Laboratory. Journal of Agroecotechnology 2 (2): 607-620. ISSN 2337-6597.
[20] Kaszak, BD. 2014. Cordyceps fungi as natural killers, new hopes for medicine and biological control factors. Ann. Parasitol 60 (3): 151-158.
[21] Koto, RG. 2013. In Vitro Digestibility of Dry Ingredients and Organic Ingredients of Maize Straw (Zea mays) Inoculated with Trichoderma sp On Different Different Incubations. 8-15. Essay.
[22] Latifian, M, Rad, B, Amani, M, Rahkhodaei, E. 2013. Mass Production of Entomopathogenic Fungi Beauveria bassiana (Balsamo) by Using Agricultural Products Based on Liquid- Solid Diphasic Method for Date Palm Pest Control. International Journal of Agriculture and Crop Sciences 5 (9): 23337-2341.
[23] Manurung, EM, Tobing, MC, Lubis, L and Priwiratama, H. 2012. Efficacy of Metarhizium anisopliae Formulations Against Oryctes rhinoceros L. Larvae (Coleoptera: Scrabaeidae) at the Insectarium. Journal of Agroecotechnology 1 (1): 47-63.
[24] Mudroncekova, S., Mazan, M., Nemcovic, M., Salamon, I. 2013. Entomopathogenic Fungus Species Beauveria bassiana (Bals.) and Metarhizium anisopliae (Metsch.) Used as Mycoinsecticide Effective in Biological Control of Ips Typographus (L.). Journal of Microbiology, Biotechnology and Food Sciences 2 (6): 2469-2472.
[25] Mustafa, Z. 2011. Effect of Trichoderma spp Application on Rhizoctonia solani Bee Disease in Robusta Seedlings. Thesis.6-9.
[26] Niken. 2009. Trichoderma Viridae Know More. http://ayyaa.multiply.com/journal. Retrieved January 10, 2013.
[27] Pahan, 2008. Complete Guide to Palm Oil. Self-Helping Spreader, Jakarta. 410 p.
[28] PPKS Palm Oil Research Center. 2009. Technical Meeting on Oil Palm. Research Center for Oil Palm. Field
[29] Rosmini and Lasmini, S.A. 2010. Identification of Local Entomopathogen Fungi and Its Pathogenicity Level against Green Planthopper Pest (Nephotettix virescens Distant.) Tungro Virus Vector On Rice Field Crops in Donggala District. Journal of Agroland 17 (3): 207-212. ISSN: 0854 - 641X.
[30] Salbiah, D., Laoh, JH, and Nurmayani. 2013. Test Several Doses of Beauveria bassiana vuillemin Against Pest Larvae Horn Beetle Oryctes rhinoceros (Coleoptera: Scarabaeidae) On Palm Oil. Journal of Applied Science Sciences (2): 137-142. ISSN 2087-5428.4.
[31] Saputra, D.D., Mudjiono, G and Afandhi, A. 2013. Addition of Vinegar to Increase Production of Conidia, Sprout and Patogenicity of Beauveria bassiana Balsamo (Deuteromycetes: Moniliales) Fungi. Journal of Plant Pests 1 (3): 60-66.
[32] Sihombing, R.H., Oemry, S and Lubis, L. 2014. Effectiveness Test of some Entomopatogenes on Oryctes rhinoceros larvae (Coleoptera: Scrabaeidae) in the Laboratory. Journal of Agroecotechnology (2): 1300-1309. ISSN 2337-6597.4.
[33] Sodiq, M. 2009. Plant Resistance Against Pests. 55-75.
[34] Suprayogi, Marheni and Oemry, S. 2015. The Effectiveness Test of Entomopathogenic Fungus Beauveria bassiana and Metarhizium anisopliae on Green Ladybugs (Hemiptera L.) (Hemiptera, Pentatomidae) on Soybean Plants (Glycine max L.) at Green House. Journal of Agroetechnology 3 (1): 320-327.
[35] Suriadi, 2015. Effect of Entomopathogenic Fungus Trichoderma sp. against the Black Ladybug Mortality (Paraeucosmetus pallicornis Dallas) on Rice Plant. 6-8. Essay.
[36] Surtikanti and Yasin, M. 2009. Entomopatogenic Effectiveness of Beauveria bassiana Vuill. from Various Growing Media to Spodoptera litura F. (Lepidoptera: Noctuidae) in Laboratory. Proceedings of the National Seminar on Cereals. 358-362.
[37] Suziani W. 2011. Test Pathogenitas Mushrooms Metarhizium anisopliae and Mushroom Cordyceps militarist Against the Borrowing Shoot larvae (Oryctes rhinoceros) (Coleoptera; Scarabaeidae) at the Laboratory. Essay. Faculty of Agriculture, University of North Sumatra, Medan.
[38] Tampubolon DY, Y Pangestiningsih, F Zahra and F Manik. 2013. Pathogenicity Test of Bacillus thuringiensis and Metarhizium anisopliae Against Mortality Spodoptera litura Fabr (Lepidoptera: Noctuidae) at Laboratory. Journal of Agroecotechnology 1 (3): 784-791.
[39] Tarigan B, Syahrial and Tarigan MU. 2013. Effectiveness Test Beauveria bassiana and Bacillus thuringiensis Against Fireworm (Setothosea asigna Eeck, Lepidoptera, Limacodidae) at the Laboratory. Journal of Agroecotechnology 1 (4): 1439-1446. ISSN 2337-6597.
[40] Trizelia Syahrawati M, Mardiah A. 2011. Pathogenicity of several Isolate Fungi Entomopatogen Metarhizium spp. Against Egg Spodoptera litura Fabricius (Lepidoptera: Noctuidae). Entomology Association of Indonesia 8 (1): 45-54.
[41] Trizelia Armon A, Jailani H. 2015. The diversity of entomopathogenic fungi in the rhizosphere of various vegetable crops. Proceedings of Indonesian Biodiversification Society Seminar. 1. (5) .998-1004. DOI 10.13057 / psnmbi / m010 307.
[42] Utomo and Pardede. 2014. Effect of seasons on Hypobiose Haemonchuscontortus and Population Fluctuation of Sheep Digestive Nematodes in Indramayu, West Java. Proceedings of Seminar. National Research Results of Higher Education. 171-192.
[43] Yuniar, W. 2013. Screening and Identification of Selulolotok Kapang on Vermicomposting Process of Oil Palm Empty Bunches (TKKS). Thesis.4-10.
[44] Yustina Fauziah Y, and Rika S. 2012. Population Structure of Horn Beetle (Oryctes rhinoceros) in Oil Palm Plantation Area of Kenantan Village, Kampar-Riau Regency. Journal of Biogenesis 8 (2): 55-63.

Total View: 182 Downloads: 11 Page No: 158-169


Cite this Article:

MLA

Lita Nasution, Riahta Corah, Nuraida, Ameilia Zuliyanti Siregar et al."Effectiveness Trichoderma and Beauveria bassiana on Larvae of Oryctes rhinoceros On Palm Oil Plant (Elaeis Guineensis Jacq.) In Vitro". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878),vol 3, no. 1, 2018, pp.158-169 AI Publications doi:10.22161/ijeab/3.1.20

APA

Lita Nasution, Riahta Corah, Nuraida, Ameilia Zuliyanti Siregar, P.(2018).Effectiveness Trichoderma and Beauveria bassiana on Larvae of Oryctes rhinoceros On Palm Oil Plant (Elaeis Guineensis Jacq.) In Vitro. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).3(1), 158-169.10.22161/ijeab/3.1.20

Chicago

Lita Nasution, Riahta Corah, Nuraida, Ameilia Zuliyanti Siregar, P.(2018).Effectiveness Trichoderma and Beauveria bassiana on Larvae of Oryctes rhinoceros On Palm Oil Plant (Elaeis Guineensis Jacq.) In Vitro. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).3(1), pp.158-169.

Harvard

Lita Nasution, Riahta Corah, Nuraida, Ameilia Zuliyanti Siregar. 2018."Effectiveness Trichoderma and Beauveria bassiana on Larvae of Oryctes rhinoceros On Palm Oil Plant (Elaeis Guineensis Jacq.) In Vitro". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).3(1):158-169.Doi:10.22161/ijeab/3.1.20

IEEE

Lita Nasution, Riahta Corah, Nuraida, Ameilia Zuliyanti Siregar."Effectiveness Trichoderma and Beauveria bassiana on Larvae of Oryctes rhinoceros On Palm Oil Plant (Elaeis Guineensis Jacq.) In Vitro", International Journal of Environment Agriculture and Biotechnology,vol.3,no. 1, pp.158-169,2018.

Bibtex

@article { litanasution2018effectiveness,
title={Effectiveness Trichoderma and Beauveria bassiana on Larvae of Oryctes rhinoceros On Palm Oil Plant (Elaeis Guineensis Jacq.) In Vitro},
author={Lita Nasution, Riahta Corah, Nuraida, Ameilia Zuliyanti Siregar , R},
journal={International Journal of Environment Agriculture and Biotechnology},
volume={3},
year= {2018} ,
}