Evaluation for stable resistance to Stenocarpella maydis in tropical maize (Zea mays L.)

Evaluation for stable resistance to Stenocarpella maydis in tropical maize (Zea mays L.) ( Vol-3,Issue-1,January - February 2018 )

Author: Kelvin Simpasa, Herbert Masole, Langa Tembo

ijeab doi crossref DOI: 10.22161/ijeab/3.1.16

Keyword: Maize, ear rot, Stenocarpella maydis, resistance, mycotoxin, Specific combining ability (SCA).

Abstract: Maize ear rots caused by Stenocarpella maydis cause reduction in yield and quality of the maize due to the mycotoxins produced by the pathogen. Breeding for resistance is the most feasible option in managing ear rots. However, to obtain stable resistance to S. maydis has been a challenge partly due to effect of the environment and availability of different isolates. The objective of this research was therefore, to determine the effect of multiple isolate inoculations in breeding for resistance to S. maydis and to identify genotypes with stable resistance. Seven inbred lines were crosses in a 7 x 7 full diallel without reciprocals. The resultant crosses (21) and their parents (7) were planted and evaluated at two sites, Lusaka and Mpongwe, during the 2015/16 cropping season. The experiment was laid out as a randomized complete block design with 3 replications. Treatments were: (1) single inoculation with isolate A, (2) single inoculation with isolate B and (3) a multiple inoculation of two isolates AB and (4) control with no inoculation at all. The mean genotypic scores were found to be 5.52, 4.96, 5.50 and 1 for treatment 1, 2, 3 and 4 respectively. The t-test analysis revealed that treatment 1 had a higher mean disease severity score (5.52) as compared to treatment 2 (4.96) (P < 0.01). Equally mean for treatment 2 (4.96) and 3 (5.50) were significantly different (P < 0.01). However, there were no significant differences between mean disease severity score for treatment 1 and 3. This indicated that multiple isolate inoculations could give rise to inappropriate genetic information due to the possibility of antagonistic effect between isolates. The genotypes (P2 x P4) and (P3 x P6) crosses were found to have stable resistance to S. maydis. These exhibited consistent significant negative SCA effects (P< 0.05) in both locations.


[1] Chambers KR, 1988. Effect of time of inoculation on Diplodia stalk and ear rot of maize in South Africa. Plant Disease, 72: 529-531.
[2] Chilipa LN, Lungu DM, Tembo L, 2016. Multiple race inoculation as an option in breeding for resistance to C. lindemuthianium in common beans. Journal of Agriculture and Crops, 45-50.
[3] Clements MJ, Kleinshmidit CE, Pataky JK, White DG, 2003. Evaluation of inoculation techniques for Fusarium ear rot and fumonisims contamination of corn. Plant Disease, 87: 147-153.
[4] Dowswell, C., Paliwal, R., & Cantrell, R. P. 1996. Maize in the Third World. Boulder, Colorado: Westview Press.
[5] El-Badawy MEM. 2012. Estimation of genetic parameters in three maize crosses for yield and its attributes. Asian J. Crop Sci, 4: 127-138
[6] Fischer RA, Byerlee D, Edmeades ED, 2014. Crop yields and global food security: will yield increase continue to feed the world? Canberra: Australian Centre for International Agricultural Research.
[7] Gasura E, Mukasa SB, 2010. Prevelance and Implications of swee tpotato recovery from sweet potato virus disease in Uganda. African Crop Science Journal 10: 195-205.
[8] Gibson RW, Mpembe J, Alicia T, Carey EE, Mwanga RO, Seal SE, 1998. Symptoms, etiology and serological analysis of sweet potato virus diseases in Uganda. Plant Pathology 47: 95-102.
[9] Griffing B, 1956. Concept of general and specific combining ability in relation to diallel crossing systems. Australian Journal of Biological Sciences 9: 463-493.
[10] Miller JD, 2001. Factors that affect the occurrence of fumonisins. Environmental Health Perspectives, 109: 321–324.
[11] M'mboyi F, Mugo S, Mwimali M, Ambani L, 2010. Maize Production and Improvement in Sub-Saharan Africa. Nairobi: African Biotechnology Stakeholders Forum (ABSF).
[12] Mukanga, M., Derera, J., Tongoona, P., & Laing, M. D. (2011). Farmers' perceptions and management of maize ear rots and their implications in breeding for resistance. Afr. J. Agric. Res, 6, 4544-4554.
[13] Munkvold GP, 2003. Cultural and Genetic Approaches to Managing Mycotoxins in Maize. DOI: 10.1146/annurev.phyto.41.052002.095510, 41, 99-116.
[14] Munkvold GP, Desjardins AE, 1997. Fumonisins in maize. Can we reduce their occurrence? Plant Dis, 81: 556–564.
[15] Pingali PL, 2001. Meeting world maize needs: Technological opportunities and priorities for the public sector. Mexico, DF (Mexico): CIMMYT.
[16] Rossouw JD, Pretorius ZA, Silva HD, Lamkey KR. 2009. Breeding for Resistance to Stenocarpella Ear Rot in Maize. In Plant Breeding review. Hoboken: John Wiley and Sons.
[17] Rossouw JD, Van Rensburg JB, Van Deventer CS, 2002. Breeding for resistance to ear rot of maize, caused by Stenocarpella maydis (Berk) Sutton. South Africa Journal Plant Soil, 4: 188-194.
[18] Tembo L, Asea G, Gibson PT, Okori P, 2013. Resistance breeding startegy for Stenocarpella maydis and Fusarium graminearum cob rots in tropical maize. Plant Breeding, 132: 83-89.
[19] Vigier B, Reid LM, Dwyer DW, Stewart RC., Sinha JT, Butler G, 2001. Maize Resistance to Gibberella ear rot:Symptoms, deoxynivalenol and yield. Canada Journal of Plant Pathology, 23: 99-105.

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