Extraction and Quantification of Carpaine from Carica papaya Leaves of Vietnam

Extraction and Quantification of Carpaine from Carica papaya Leaves of Vietnam ( Vol-2,Issue-5,September - October 2017 )

Author: Do Thi Hoa Vien, Tran Van Loc

ijeab doi crossref DOI: 10.22161/ijeab/2.5.16

Keyword: Alkaloid, Carica papaya leaves, carpaine, extract, purify, quantitative analyze.

Abstract: Our previous research indicated that carpaine and its derivative pseudocarpaine extracted from Carica papaya leaves had anti-cancer activity. In this study, we extracted the total alkaloid from Carica papaya leaves, then extracted carpaine and quantitative analyzed carpaine in the total alkaloid. Carica papaya leaves was crushed, and then extracted with EtOH to obtain the total extract. This extract was extracted with suitable solvent to obtain total alkaloid. Continued to extract the total alkaloid by using open column chromatography and crystallizing method to purify carpaine. The research result showed that the total alkaloid in Carica papaya leaves was 0.2% comparing with dried material. Quantitative analyze of purified carpaine by HPLC determined that carpaine was the main alkaloid with the content was 63% of the total alkaloid extracted from Carica papaya leaves.


[1] H.W. Tietze (1997). Papaya the Medicine Tree. Bermagui South-Harald W. Tietze Pub...
[2] A.U. Ogan (1971). The basic constituents of the leaves of Carica papaya. Phytochemistry 10(10), 2544-2547.
[3] A. Canini (2007). Gas chromatography mass spectrometry analysis of phenolic compounds from Carica papaya L. leaves. J. of Food Composition and Analysis 20(7), 584-590.
[4] Do Tat Loi (1999). Vietnamese medicine. Medicinal editor, Hanoi - Vietnam.
[5] Do Huy Bich (2004). Medicinal herb and animal in Vietnam. Vol. 1 and 2, Editor of Science and Technique, Hanoi 2004.
[6] H. Clark (2010). Papaya leaves, the anti-cancer treatment. Cancer therapies, from: http://www.huldaclarkzappers.com/?page_id=134.
[7] N. Otsuki, N.H. Dang, E. Kumagai, A. Kondoc, S. Iwataa, C. Morimotoa (2010). Aqueous extract of Carica papaya leaves exhibits anti-tumor activity and immunomodulatory effects. Journal of Ethno pharmacology 127 (3), 760-767.
[8] Do Thi Hoa Vien, Do Thi Thao (2013). Preliminary findings on anticancer and lymphocyte stimulated activities of bioactive compounds extracted from Vietnam Carica papaya leaves. Journal of Food Science and Engineering 3, 447-452.
[9] Ho Thi Ha (2014). Research on biological activity of the compounds extracted from Carica papaya leaves. Thesis of Doctor Philosophy, Hanoi University of Science and Technology, Hanoi, Vietnam.
[10] Singh I.D. (1978). Papaya. Oxford and IBH publishing co. PVT.LTD, New Delhi Bombay, Calcutta.
[11] Tasqiah Julianti (2014). Discovery of natural antiprotozoals from medicinal plants Saussure Costus and Carica papaya. Thesis of Doctor Philosophy, University of Basel, Switzerland, 41-48.
[12] Taro Sato, Sakae Aoyagi, Chihiro, Kibayashi (2003). Enantionselective total synthesis of (+)-Azimine and (+)-Carpaine. Org. Lett., 5(21), 3839-3842.
[13] Pedro Chávez – Quintal, Tania González – Flores, Ingrid Rodríguez – Buenfil, Santiago Gallegos – Tintoré (2011). Antifungal activity in ethanolic extracts of Carica papaya L. cv. Maradol leaves and seeds. Indian J Microbial, 51(1), 54-60.
[14] Tichý M. and ¬Sicher J. (1962). The configuration of carpaine. Tetrahedron letters, No. 12, 511-514.

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