Germination Characters as Affected by Salinity Stress and Soaking Grain Sorghum Genotypesin Humic acid

Germination Characters as Affected by Salinity Stress and Soaking Grain Sorghum Genotypesin Humic acid ( Vol-2,Issue-6,November - December 2017 )

Author: Kandil A.A., A.E. Sharief, Doha E. A. Elbadry

ijeab doi crossref DOI: 10.22161/ijeab/2.6.63

Keyword: Salinity Stress, Genotypesin Humic acid, Mansoura University.

Abstract: In order to investigate salinity stress on sorghum germination indices, an experiment conducted in the Faculty of Agriculture, Mansoura University from June and July 2017 in Agronomy Department, Seed Science Laboratory. The goals of the investigation aimed to screening for five (Sorghum bicolor L.(Moench) cultivarsviz. Dorado, hybrid 306, Giza 15, Mecca hybrid and H-305 under salinity stress, sodium chloride (Na Cl) at the levels of 0 (as control), 3, 6, 9, 12 and 15 dS/m-1 and soaking in humic acid. The results showed that soaking seed in humic acid exceeded percentage of germination, germination rate, germination index, energy of germination and chlorophyll content by 5.2, 7.7, 17.1, 65.8 and 17.8 %, respectively. The highest germination percentage (91.9 %), germination rate (3.08), germination energy (59.7), seedling vigor index (1483.7) and chlorophyll content (2.88) were obtained from sown Mecca hybrid. The maximum germination index (117.22) was obtainedfrom sown Giza 15 cultivar.The results point out that cumulative salinity level from 3 to 15 dSm-1condensedall studied germination characters. Accumulative salinity levels to 15 dSm-1condensedpercentage of germination, germination rate, index of germination, germination energy and seedling vigor index by 15.9, 15.0, 30.0, 35.9 and 37.6 %, respectively compared without salinity application. It could recommended that soaking sorghum seed of Meeca hybrid with humic acid for 12 h under salinity of concentration of 6 dSm-1 enhanced germination characters compared with other cultivars and salinity concentrations, it mean cultivated it under reclaimed saline soil in Egypt.


[1] Abdel-Baki, A. A. and J.D. Anderson 1970. Viability and leaching of sugars from germinating barley. Crops Science, 10: 31 – 34.
[2] Ahmed, S. 2009. Effect of soil salinity on the yield and yield components of Mungbean. Pakistan J. Bot., 4(1):263-268.
[3] Aishah, H. S., A. R. Saberi, R. A. Halim and A. R. Zahara 2010. Salinity Effects on Germination on Forage Sorghum. Journal of Agronomy 9(4): 169-174. DOI:10.3923/ja.2010.169.174
[4] Almodares, A., M. R. Hadi, B. Kholdibarien, B. Samedani and Z. A. Kharazian 2014. The Response of Sweet Sorghum Cultivars to Salt Stress and Accumulation of Na+, Cl-, K+ ions in relation to Salinity. Journal of Environmental Biology, 35:733-739.
[5] Almodares, A., M.R. Hadi and B. Dosti 2007. Effect of Salt Stress on Germination Percentage and Seedling Growth in Sweet Sorghum Cultivars. J. of Biological Sciences, 7(8): 1492-1495.
[6] Ali, M.A., K. Jabran, S.I. Awan, A. Abbas, M. Ehsanullah, Zulkiffal, T. Acet, J. Farooq and A. Rehman 2011: Morpho-physiological diversity and its implications for improving drought tolerance in grain sorghum at different growth stages. Australian Journal of Crop Science 5(3): 311-320.
[7] Ali, H., Y. Akbar, Dr. Abdul Razaq and D. Muhammad 2014. Effect of humic acid on root elongation and percent seed germination of wheat seeds. International Journal of Agriculture and Crop Sciences, 7(4): 196-201.
[8] Asenjo, M.C., J.L. Gonzales and J.M. Maldonado, 2000. Influence of humic extracts on germination and growth of ryegrass. Communications Soil Sci. Plant Anal., 31: 101-114.
[9] Asfaw, K.G. 2011. Effects of Salinity on Seedling Biomass Production and Relative Water Content of Twenty Sorghum (Sorghum biolor L. Moench) Accessions. Asian Journal of Agricultural Sciences, 3(3): 242-249.
[10] Behzadnejad, J. and E. Tohidinejad 2014. Ameliorative Effects of Exogenous SA on Germination of Sorghum under Salinity Stress. Journal of Applied Science and Agriculture, 9(4): 1519-1524.
[11] Çavuşoğlu, K. and H.G. Ergin 2015. Effects of humic acid pretreatment on some physiological and anatomical parameters of barley (Hordeumvulgare l.) Exposed to salt stress. Bangladesh J. Bot. 44(4): 591-598.
[12] Chauhan, R. R., C. Reema, S. Alka and P.K. Singh 2012. Salt Tolerance of Sorghum bicolor Cultivars during Germination and Seedling Growth. Research Journal of Recent Sciences, 1(3):2-10.
[13] Dadar, A., A. Asgharzade, and M. Nazari 2014. Investigation effects of different salinity levels on sorghum Bicolor seed germination characters. Indian J. Sci. Res. 7 (1): 1031-1034.
[14] De oliveir, A. B. and E. Gomes-Filho 2009. Germination and Vigor of Sorghum Seeds Under Water and Salt Stress. RevistaBrasileira de Sementes, 31(3):.048-056.
[15] Ellis, R.A. and E.H. Roberts 1981. The quantification of ageing and survival in orthodox seeds. Seed Sci. Technol., 9: 373-409.
[16] El-Naim, A.M., K. E. Mohammed, E. A. Ibrahim and N. N. Suleiman 2012. Impact of Salinity on Seed Germination and Early Seedling Growth of Three Sorghum (Sorghum biolor L. Moench) Cultivars. Science and Technology, 2(2): 16-20.
[17] Geressu, K. and M. Gezahagne, 2008. Response of some lowland growing sorghum (Sorghum bicolor L. Moench) accessions to salt stress during germination and seedling growth. African Journal Agriculture Research, 3(1): 044-048.
[18] Gomez, K.A. and A.A. Gomez 1991. Statistical Procedures in Agricultural Research, John Wiley and Sons, New York 2nd edition, pp. 680.
[19] Hefny, M.M., E. M. R. Metwali and A. I. Mohamed 2013. Assessment of genetic diversity of sorghum (Sorghum bicolor L. Moench) genotypes under saline irrigation water based on some selection indices. Australian Journal of Crop Science, 7(12): 1935-1945.
[20] Hussain, S. H, A. Khaliq, A. Matloop, W.M. Ashfaq and I. Afzal 2013. Germination and growth response of three wheat cultivars to NaCl salinity.Soil & Environment, 32(1): 36-43.
[21] ISTA Rules. (2013). Germination Sec. Chapter 5: pp. 5 – 44.
[22] Jamil, M., D.B. Lee, K.Y. Jung, M. Ashraf, S.C. Lee and S.E. Rha 2006. Effect of salt (NaCl) stress on germination and early seedling growth of four Vegetable species.Journal Central European Agriculture, 7(2): 273-282.
[23] Jajarmi, V. 2007. Effects of Water Stress on Germination Indices in Ten Rapeseed Cultivars (Brassica napus L.). Abstracts, The Second Seminar of Agriculture and Environment. Islamic Azad University, Khoy Branch, Iran.
[24] Kandil A.A., A.E. Sharief, W.A.E. Abido and M.M. Ibrahim 2012. Effect of Salinity on Seed Germination and Seedling Characters of Some Forage Sorghum Cultivars. International Journal of Agriculture Sciences, 4(7): 306-311.
[25] Karim, M. A.; N. Utsunomiya and S. Shigenaga 1992. Effect of sodium chloride on germination and growth of hexaploid triticale at early seedling stage. Japanese Journal of Crop Science,61: 279 – 284.
[26] Kausar, A., M. Y. Ashraf, I. Ali, M. Niaz and Q. Abbass 2012. Evaluation of sorghum varieties/lines for salt tolerance using Physiological indices as screening tool. Pak. J. Bot., 44(1): 47-52.
[27] Khan, A., M. Ibrar and I. Ahmad 2014. A preliminary approach to halo sensitivity of sorghum cultivars. African Journal of Plant Science, 8(1): 76-83.
[28] Mohammadi, N. and M. Mojaddam 2014. The Effect of Water Deficit Stress on Germination Components of Grain Sorghum Cultivars. Indian Journal of Fundamental and Applied Life Sciences, 4(4): 284-291.
[29] Munns, R., James, R.A. and A. Lauchli 2006. Approaches to increasing the salt tolerance of wheat and other cereals. J. Exp. Bot., 57: 1025-1043.
[30] Nazi, F., H. Reza and R. Rahman 2014. Effect of Humic Fertilizer on Germination of Wheat Seeds under Drought Stress. Advances in Bioresearch 5(4): 98-102.
[31] Noreen, S. and M. Ashraf 2008. Alleviation of adverse effects of salt stress on sunflower (Helianthus annuus L.) by exogenous application of salicylic acid: Growth and photo-synthesis. Pakistan Journal Botany, 40(4): 1657-1663.
[32] Ruan, S., Q. Xue and K. Tylkowsko 2002. The influence of priming on germination of rice (oryza sativa L.) seeds and seedling emergence and in flooded performance soils. Seed Sci. Technil., 30: 61– 67.
[33] Russell, D.F. 1986. MSTAT-C computer based data analysis software Crop and Soil Science Department, Michigan State University USA.
[34] Sam, A., A. Y. Edris and M.S.A. Abo 2014. Effect of Salinity on Seed Germination and Seedling Growth of Pearl millet (Pennisetumglaucum L.) and Sorghum (Sorghum bicolor L.), Journal of Plant and Pest Science, 1 (1): 01-08.
[35] Saroj, M. and D. Soumana, 2014. Salt stress induced changes in growth of germinating seeds of Vigna mungo(L.) Hepper and Vigna aconitifolia (Jacq.) Marechal. IOSR Journal of Agriculture and Veterinary Science, 7. (4):44-48.
[36] Sawamery, N. and M. Mojaddam 2014. The Effect of Salinity Stress on Germination Components of Grain Sorghum Cultivars. Indian Journal of Fundamental and Applied Life Sciences, 4(4): 431-437.
[37] Siddig, A.A.M. and A.A.Y. Idris 2015. Response of Sorghum (Sorghum bicolor L.) Cultivars to Salinity Levels at Early Growth Stages. J. of Agricultural Science and Engineering, 1(1): 11-16.
[38] Snedecor GW and WG. Cochran 1980. Statistical Methods. 7ThEd. Iowa State University Press, Iowa, USA, ISBN-10: 0-81381560-6, Pp: 507.
[39] Sun, Y., G. Niu1, P. Osuna, L. Zhao, G. Ganjegunte, G. Peterson, J. R. Peralta-Videa, and J. L. Gardea-Torresdey 2014. Variability in Salt Tolerance of Sorghum bicolor L. Agricultural Science, 2(1): 9-21.
[40] Tabatabaei, S. A., and A. Anagholi 2012. Effects of salinity on some characteristics of forage sorghum genotypes at germination stage. International Journal of Agriculture and Crop Sciences, 4(14): 979-983.
[41] Tigabo, E., M. Andargie and K. Tesfaye 2013. Genotypic Variation for Salinity Tolerance in Sorghum (Sorghum bicolor (L.) Moench) Genotypes at Early Growth Stages. Journal of Stress Physiology & Biochemistry, 9 (2): 253-262.
[42] Zafar, S., M. Y. Ashraf, M. Niaz1, A. Kausar and J. Hussain 2015. Evaluation of wheat genotypes for salinity tolerance using Physiological indices as screening tool. Pakistan J. Botany, 47(2): 397-405.
[43] Wei G. and W. Qing-Xiang 2011. Effects of seed soaking with humic acid on wheat seedlings antioxidant system under salt-alkali stress. YingyongShengtaiXuebao, 22 (10):2539.

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