Identifying QTLs Associated and Marker-Assisted Selection for Salinity Tolerance at the Seedling, Vegetative and Reproductive Stages in Rice (Oryza Sativa L.)

Identifying QTLs Associated and Marker-Assisted Selection for Salinity Tolerance at the Seedling, Vegetative and Reproductive Stages in Rice (Oryza Sativa L.) ( Vol-2,Issue-6,November - December 2017 )

Author: Nguyen Thi Lang, Nguyen Trong Phuoc, Pham Thi Thu Ha, Bui Chi Buu

ijeab doi crossref DOI: 10.22161/ijeab/2.6.20

Keyword: Seedling, reproductive stage, salinity, SSR, QTL, vegetative stage.

Abstract: Salinity affects rice growth in all growth stages, with the seedling and reproductive stages being the most sensitive. Genetically improving salt tolerance of rice is an important objective of rice breeding programs. Hence, mapping quantitative trait loci (QTL) will be useful for marker-assisted selection in rice breeding programs. An advanced backcross population (BC2F2) was developed with the parents included OM5629 as a donor of salt tolerance and OM7347 as a recurrent parent with good quality traits and drought tolerance. Molecular markers associated with both qualitative and quantitative trait loci (QTL) salt tolerance were identified by using 416 polymorphic SSR markers. QTLs, associated with stress tolerance at EC = 15 dS/m at seedling stage, detected from the BC2F2 population of OM7347/OM5629, were located on chromosomes 1 and 3. Three QTLs were identified at the intervals of RM3252-S1-1 - RM10694, RM3740-RM5336 and RM11125-RM9 with genetic distance of 4.4, 4.5 and 18 cM on chromosome 1, respectively. Two QTLs at the intervals of RM3867-RM6959 and RM6876-RM4425 with genetic distance of 4.5 and 18.0 cM on chromosome 3, respectively. One QTL on chromosome 5 was detected at the interval of RM874 - RM10359, it was associated with salt stress tolerance under EC = 8dS/m at vegetative stage. Three QTLs at the regions of RM1324-RM2412, RM1185-RM24, and RM1282-RM2560 on chromosome 1, and one QTL of RM453-RM511 on chromosome 12, were related to salt tolerance under EC = 8dS/m at reproductive stage. Two tightly linked markers as RM3252-S1-1 and RM3867, were exhibited their effectiveness in identification of salt tolerance genotypes in BC3F6 population of OMCS2000/ Pokkali. The identification of new QTLs associated with salt tolerance will provide important information for the functional analysis of rice salinity stress.

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