In Vitro screening of larvicidal and insecticidal activity of methanolic extracts of Artocarpus heterophyllus, Artocarpus altilis and Piper betle

DOI: 10.22161/ijeab/2.1.37

Keyword: Insecticidal, larvicidal, Bruchus pisorum, Tribolium castaneum, Sitophilus oryzae, Drosophila melanogaster.

Abstract: The aim of this work was to evaluate the larvicidal and insecticidal activity of the selected plants namelyArtocarpus altilis, Piper betle and Artocarpus heterophyllus. The leaves of Artocarpus altilis and Artocarpus heterophyllus and roots of Piper betle were subjected to methanolic solvent extraction for the isolation of various bioactive constituents. The evaluation of larvicidal activity was carried out using late third instar larvae of Drosophila melanogaster. The insecticidal activity of extracts was studied against adult Bruchus pisorum, Tribolium castaneum, Sitophilus oryzae and was evaluated by direct contact application method .Nucleic acids and protein contents are regarded as important biomarkers of the metabolic potential of cells, as these play the main role in regulating the different activities of cells. Piper betle and Artocarpus heterophyllusextractshad a reducing effect on the nucleic acid and protein content in the larvae in a dose dependent manner whereas Artocarpus altilis extract did not exhibit any significant larvicidal activity. Piper betle and Artocarpus heterophyllusextractsshowed good insecticidal activity whereas A.altilis extract showed poor insecticidal activity. The results of the present study clearly indicate that Piper betle and Artocarpus heterophyllus extracts can be developed as ecofriendly larvicides and were also quite effective as insecticides for providing a better and excellent alternate for the control of insects.

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Sindhu S Nair, Vaibhavi Kavrekar et al."In Vitro screening of larvicidal and insecticidal activity of methanolic extracts of Artocarpus heterophyllus, Artocarpus altilis and Piper betle". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878),vol 2, no. 1, 2017, pp.281-288 AI Publications doi:10.22161/ijeab/2.1.37

Sindhu S Nair, Vaibhavi Kavrekar, P.(2017).In Vitro screening of larvicidal and insecticidal activity of methanolic extracts of Artocarpus heterophyllus, Artocarpus altilis and Piper betle. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).2(1), 281-288.10.22161/ijeab/2.1.37

Sindhu S Nair, Vaibhavi Kavrekar, P.(2017).In Vitro screening of larvicidal and insecticidal activity of methanolic extracts of Artocarpus heterophyllus, Artocarpus altilis and Piper betle. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).2(1), pp.281-288.

Sindhu S Nair, Vaibhavi Kavrekar. 2017."In Vitro screening of larvicidal and insecticidal activity of methanolic extracts of Artocarpus heterophyllus, Artocarpus altilis and Piper betle". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).2(1):281-288.Doi:10.22161/ijeab/2.1.37

Sindhu S Nair, Vaibhavi Kavrekar."In Vitro screening of larvicidal and insecticidal activity of methanolic extracts of Artocarpus heterophyllus, Artocarpus altilis and Piper betle", International Journal of Environment Agriculture and Biotechnology,vol.2,no. 1, pp.281-288,2017.

@article { sindhusnair2017in,
title={In Vitro screening of larvicidal and insecticidal activity of methanolic extracts of Artocarpus heterophyllus, Artocarpus altilis and Piper betle},
author={Sindhu S Nair, Vaibhavi Kavrekar , R},
journal={International Journal of Environment Agriculture and Biotechnology},
volume={2},
year= {2017} ,
}