Isolation and identification of microbial and fungal flora from female hair samples in Riyadh Saudi Arabia

Isolation and identification of microbial and fungal flora from female hair samples in Riyadh Saudi Arabia ( Vol-3,Issue-1,January - February 2018 )

Author: Salma A. Alghamdi, Haya A. Alotaibi, Munira Z. Al-Subai, Prof. Suaad S. Alwakeel

ijeab doi crossref DOI: 10.22161/ijeab/3.1.1

Keyword: keratinophilic fungi, microbial and fungal flora,female hair.

Abstract: The human hair harbors several species of fungi and also bacteria. The present study was performed to determine the prevalence of keratinophilic fungi and bacteria from hair samples of femalesfrom November 2016 to April 2017. A total of 50 human hair samples were examinedusing hair-baiting techniques for isolation. After the incubation period, the number of colony forming unit was counted. The microorganisms were identified based on the colony morphology from culture and microscopic features. After purification, each representative colony was gram-stained and examined for cell morphology and gram reaction under a light microscope. Fungal isolates included were Aspergillus niger, Aspergillus flavus, Penicilliumspp, Alternaria alternata, Chrysosporium keratinophilum. Cladosporium cladosporioides and Trichosporon mucoides. Isolated bacterial species included gram positive bacteria such as Leuconostoc mesenteroidess spcremoris, Kocuriarosea, Staphylococcus haemolyticus, and the gram negative bacteria Kocurikristinae, Stenotrophomonas maltophilia, and Micrococcus luteu/ lylae. Human hair samples from females studied were found have several fungal and bacterial isolates, some of which can cause some serious disease in humans. Health


[1] Albanese, A., Spanu, T., Sali, M., Novegno, F., D'Inzeo, T., Santangelo, R., Mangiola, A., Anile, C. and Fadda, G., 2006. Molecular identification of Leuconostocmesenteroides as a cause of brain abscess in an immunocompromised patient. Journal of clinical microbiology, 44(8), pp.3044-3045.
[2] Ali, Z.M. and Majid, Z., 2008. Isolation of dermatophytes and related keratinophilic fungi from the two public parks in Ahvaz.Jundishapur Journal of Microbiology, 2008(1, Winter), pp.20-23.
[3] Ali-Shtayeh MS, Salameh AAM, Abu-Ghdeeib SI, Jamous RM. .2001 .Hair and scalp mycobiota in school children in Nablus area. Mycopathologia;150:127–135.
[4] Anbu P, Hilda A, Gopinath SCB, 2004. Keratinophilic fungi of poultry farm and feather dumpling soil in Tamil Nadu, India. Mycopathologia, 158:303-309.
[5] Atchade, E., Jean-Baptiste, S., Houzé, S., Chabut, C., Massias, L., Castier, Y., Brugière, O., Mal, H. and Montravers, P., 2017. Fatal invasive aspergillosis caused by Aspergillus niger after bilateral lung transplantation. Medical mycology case reports.
[6] Chen, H.M., Chi, H., Chiu, N.C. and Huang, F.Y., 2015.Kocuriakristinae: a true pathogen in pediatric patients. Journal of Microbiology, Immunology and Infection, 48(1), pp.80-84.
[7] Chepchirchir A. Bii C. and Ndinya J.O (2009) Dermatophyte infections in primary school children in Kibera slums of Nairobi East African Medical Journal, 86 no. 2 pp. 67- 70.
[8] Deshmukh, S.K., Verekar, S.A. and Shrivastav, A., 2010. The occurrence of keratinophilic fungi in selected soils of Ladakh (India).Natural Science, 2(11), pp.1247-1252.
[9] Dignani, M.C., Grazziutti, M. and Anaissie, E., 2003.Stenotrophomonas maltophilia infections. In Seminars in respiratory and critical care medicine (Vol. 24, No. 01, pp. 089-098). Copyright© 2002 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA. Tel.:+ 1 (212) 584-4662.
[10] De Bonis P1, Albanese A, Lofrese G, de Waure C, Mangiola A, Pettorini BL, Pompucci A, Balducci M, Fiorentino A, Lauriola L, Anile C, Maira G. Neurosurgery. 2011 Oct Postoperative infection may influence survival in patients with glioblastoma: simply a myth? ;69(4):864-8; discussion 868-9. doi: 10.1227/NEU.0b013e318222adfa.
[11] Ellis, D., Davis S., Alexiou H., Handke R. and Bartley R, 2007. Description of medical fungi, Mycology Unit, Women`s and Children`s Hospital, University of Adelaide , Australia (198 pages).
[12] Falcone, M., Campanile, F., Giannella, M., Borbone, S., Stefani, S. and Venditti, M., 2007. Staphylococcus haemolyticus endocarditis: clinical and microbiologic analysis of 4 cases. Diagnostic microbiology and infectious disease, 57(3), pp.325-331.
[13] Ganaie, M.A., Sood, S., Rizvi, G. and Khan, T.A., 2010. Isolation and identification of keratinophilic fungi from different soil samples in Jhansi City (India).Plant Pathology Journal (Faisalabad), 9(4), pp.194-197.
[14] Gherbawy YA, Maghraby TA, El-Sharony HM, Hussein MA. Mycobiology. 2006 Dec Diversity of keratinophilic fungi on human hairs and nails at four governorates in Upper Egypt. Mycobiology, 34(4):180-4.
[15] Jappe U. Schroder K, Zillikens D, Petzoldt D., 2003. Tuft hair folliculitis associated with pemphigus vulgaris. Journal of European Aacademy of Dermatology and Venereology, 17(2), pp.223-236
[16] Kannan,P., Janak,C. and Selvi, G, 2006. Prevalence of dermatophytosis and other fungal agents isolated from clinical samples. Indian Journal of Medical Microbiology, 24:(3) 212-215.
[17] Kim, J.D., 2007. Purification and characterization of a keratinase from a feather-degrading fungus, Aspergillus flavus Strain K-03.Mycobiology, 35(4), pp.219-225.
[18] Lange-Asschenfeldt, B., Marenbach, D., Lang, C., Patzelt, A., Ulrich, M., Maltusch, A., Terhorst, D., Stockfleth, E., Sterry, W. and Lademann, J., 2011.Distribution of bacteria in the epidermal layers and hair follicles of the human skin.Skin pharmacology and physiology, 24(6), pp.305-311.
[19] Lee, M.J., Park, J.S., Chung, H., Jun, J.B. and Bang, Y.J., 2011.Distribuition of soil keratinophilic fungi isolated in summer beaches of the east sea in Korea. Korea J Med Mycol, 16, pp.44-50.
[20] Mahmoudabadi AZ, Zarrin M. Jundishapur J. of Microbiol., 2008, 1(1): 20-23.
[21] Mase, K., Hasegawa, T., Horii, T., Hatakeyama, K., Kawano, Y., Yamashino, T. and Ohta, M., 2000.Firm adherence of Staphylococcus aureus and Staphylococcus epidermidis to human hair and effect of detergent treatment.Microbiology and immunology, 44(8), pp.653-656.
[22] Nakagawa Y, Shimazu K, Ebihara M, Nakagawa K. J. Infect. Chemother., 1999.5:97-100.
[23] Oda, M., Saraya, T., Wakayama, M., Shibuya, K., Ogawa, Y., Inui, T., Yokoyama, E., Inoue, M., Shimoyamada, H., Fujiwara, M. and Ota, T., 2013. Calcium oxalate crystal deposition in a patient with Aspergilloma due to Aspergillus niger. Journal of thoracic disease, 5(4), p.E174.
[24] Purty, S., Saranathan, R., Prashanth, K., Narayanan, K., Asir, J., Devi, C.S. and Amarnath, S.K., 2013. The expanding spectrum of human infections caused by Kocuria species: a case report and literature review. Emerging microbes & infections, 2(10), p.e71.
[25] Shadzi S, Chadeganipour M, Alimoradi M. Isolation of keratinophilic fungi from elementary schools and public parks in Isfahan, Iran. Mycoses 2002; 45: 496-499.
[26] Sharma, R. and Rajak, R.C., 2003. Keratinophilic fungi: Nature’s keratin degrading machines! Resonance, 8(9), pp.28-40.
[27] Shukia P, Shukla CB, Kango N, Shukla A., 2003. Isolation and characterization of a dermatophyte, Microsporumgypseum from poultry farm soils of Rewa (Madhya Pradesh), India. Pakistan Journal of Biological Sciences,6: 622-625.
[28] Filipello Marchisio, L. Preve, and V. Tullio, “Fungi responsible for skin mycoses in Turin (Italy),” Mycoses, vol. 39, no. 3-4, pp. 141–150, 1996.
[29] World Health Organization, 2005.Epidemiology and management of common skin diseases in children in developing countries.WHO/FCH/CAH / 05.12.
[30] Zarrin, M. and Haghgoo, R., 2011.Survey of keratinophilic fungi from soils in Ahvaz, Iran. Jundishapur Journal of Microbiology, 4(3), pp.191-194.

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