Mass Culturing of Stem and Bulb Nematode (Ditylenchus dipsaci) for use in screenİng and İmpression Training on Carrot Discs

Mass Culturing of Stem and Bulb Nematode (Ditylenchus dipsaci) for use in screenİng and İmpression Training on Carrot Discs ( Vol-2,Issue-6,November - December 2017 )

Author: Tohid Behmand, Lerzan Ozturk, İbrahim Halil Elekcioğlu

ijeab doi crossref DOI: 10.22161/ijeab/2.6.48

Keyword: Resistant,stem and bulb nematode,culture,Technique.

Abstract: Stem and bulb nematode, Ditylenchus dipsaci, is a very important plant parasitic nematode, has a very wide host range and causes economic yield losses in many cultural plants in worldwide. Its races are very diverse and found in most temperate areas of the world. D. dipsaci is a migratory endoparasite, has the ability to enter into a dormancy stage. Genetic resistance offers one of the best control methods within the integrated pest management strategies for D. dipsaci. However mass rearing of stem and bulb nematode requires for the resistant studies including screening for this nematode species. The aim of study is focused on alternative rearing methods using carrot discs as a food source, culturing medium and provide a clearly outlined and visually informative guide. Carrot discs enable the rearing of high numbers of individuals of D. dipsaci for timely use in experiments and for screening purposes in under sterile conditions to provide a clean, same and pure source of inoculum. The carrot disc method has been shown to be suitable for stem and bulb nematode multiplication.


[1] Bingefors, S., and K. B. Eriksson. 1963. Rearing stem nematode inoculum on tissue culture: Preliminary report. Landbrukshogskolan Annaler 29: 107-118.
[2] Huettel, R. N. 1985. Carrot disc culture. Pp. 212 in B. M. Zuckerman, W. F. Mai, and M. B. Harrison, eds. Plant nematology, laboratory manual. University of Massachusetts Agricultural Experiment Station, University of Massachusetts, Amherst.
[3] Krusberg, L. R. 1961. Studies on the culturing and parasitism of plant-parasitic nematodes, in particular Ditylenchus dipsaci and Aphelenchoides ritzemabosi on alfalfa tissues. Nematologica 6:181-200.
[4] Lauritis, J. A., R. V. Rebois, and L. I. Graney. 1982. Gnotobiotic cultivation of Heterodera glycines Ichinohe on Glycine max (L.) Merr. Journal of Nematology 14:422-424..
[5] Lawn, D. A., and G. R. Noel. 1984. Monoxenic culture of Pratylenchus scribneri on carrot disks. Proceedings of the First International Congress of Nematology. P. 47 (Abstr.)
[6] Myers, R. J., W. A. Feder, and P. C. Hutchens. 1965. The rearing ofRadopholus similis (Cobb) Thorne on grapefruit, okra, and alfalfa root callus tissues. Proceedings of the Helminthological Society of Washington 32:94-95
[7] Moody, E. H., B. F. Lownsbery, and J. M. Ahmed. 1973. Culture of the root-lesion nematode Pratylenchus vulnus on carrot disks. Journal of Nematology 5:225-226.
[8] O'Bannon,J. H., and A. L. Taylor. 1968. Migratory endoparasitic nematodes reared on carrot discs. Phytopathology 58:385.
[9] Young, T. W. 1954. An incubation method for collecting migratory endo-parasitic nematodes. Plant Disease Reporter 38:794-795

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