Morphological and physiological variation among different isolates of Alternaria spp. from Rapeseed-Mustard

Morphological and physiological variation among different isolates of Alternaria spp. from Rapeseed-Mustard ( Vol-2,Issue-5,September - October 2017 )

Author: Rufaida Monowara, Nazmoon Naher Tonu, Fatema Begum, Md. Masud Karim , Nazneen Sultana

ijeab doi crossref DOI: 10.22161/ijeab/2.5.21

Keyword: Alternaria brassicae, mustard, morphology, physiology, culture media, variability.

Abstract: To find out the Morphological variation on growth and sporulation of Alternaria species of Alternaria leaf blight of mustard from 10 representative geographical locations of Bangladesh, this experiment was conducted at Plant Pathology Laboratory, Oilseed Research center, Bangladesh Agricultural Research Inistitute (BARI), Joydevpur, Gazipur, Bangladesh. All the isolates showed high level of variability in in-vitro in respect of radial mycial growth, colony colour, sub surface colour, colony shape, colony texture, zonation (surface and sub surface), length and width of conidia, beak length and number of septa. The maximum and minimum radial mycial growth was recorded 90 mm in isolate NATAb and 83.67 mm in isolate GAZAb, respectively at 14 days after incubation. Significant variation in conidial length, width, beak and no. of conidia observed in all isolates. The length of conidia ranged from 41.56 to 117.54µm with 3 to 11 transverse and 0 to 3 vertical septa. The width and beak length varied from 10.34 to 23.12 µm and 16.78 to 72.65 µm ,respectively. Surface colour were olivacious green to black and circular shaped colonies were observed in all isolates on PDA medium. Colony texture were cottony to velvety. Subsurface colour varied from light brown to black and pinkish. Zonation found in some isolates and some did not produce on both surface and subsurface. All conidia were murifrom and light brown to deep brown in colour. Potato Carrot Dextrose Agar medium (PCDA) and 25 o C temperature were found optimum for different isolates for mycelial growth and sporulation.


[1] Aneja J. K. and Agnihotri A., (2013), Alternaria blight of oilseed brassicas: epidemiology and disease control strategies with special reference to use of biotechnological approaches for attaining host resistance. J. of Oils.Bras. , 4(1):1-10.
[2] Anju M, Rajib R and Jagatpati T;(2013); Alternaria pathogenicity and its strategic controls; Res. J. of Biol.; 1: 01-09.
[3] Barry M. Pryor and Themis J. Michailides, (2002), Morphological, Pathogenic, and Molecular Characterization of Alternaria Isolates Associated with Alternaria Late Blight of Pistachio, Phytopah, 92(4), 406-416.
[4] BBS, (2015). Year Book of Agricultural Statistics of Bangladesh, 2014-15. Statistics Division, Ministry of Planning, Dhaka.
[5] Chand G and Chandra K. K.(2014), Symptomological, Cultural and Molecular Variability of Alternaria brassicicola Leaf Spot in BroccoliI (Brassica oleracea var. Italica L.), Int J Pharm Bio Sci; l 5(2), (B) : 680 – 688.
[6] Deep. S, Sharma P., Behera N and Chowdappa P,(2014),Diversity in Indian Isolates of Alternaria brassicicola(Schwein) Wiltshire Causing Black Leaf Spot Disease in Cauliflower, Plant Pathol J., 13(4):232-245.
[7] Degenhardt K. J., Sxonopeo W. P., and Kondra Z. P. ( 1974), Effects of Alternaria Black spot on Yield Oil content and Protein content of Rapeseed. Can. J. Plant Sci. 54 (4): 795-799.
[8] Duczek, L. J., Seidle, E., Reed, S. L., Sutherland, K. A., Rude, S. V. and Rimmer, S. R. (1999). Effect of swathing on alternaria black spot in Brassica rapa canola in Saskatchewan. Can. J. Plant Sci. 79: 299–302.
[9] Ellis.M.B,(1971),Dematiaceous Hyphomycetes, Commonwealth Mycological Inistitute,Kew,Surry,CABI Publishing,Edition-1,pp 482.
[10] Fakir, G.A. (2008), Development of model for Alternaria leaf blight of Mustard in Bangladesh. APM workshop Held on 11-14 Feb at Dhaka, Bangladesh Science Foundation. Uttara, Dhaka. pp44.
[11] Freed R.D, Scott .D.E, (1986). MSTATC Crop and Soil Science Department, Michigan State University, MI, USA.
[12] Ghasemi M., Aghajani M. A., Faraji A. and Nejad M. R.S., (2013), Relationship Between Incidence AND Severity of Alternaria Blight Disease On Different Species of Brassicae in Gonbad Region, Iran. J. Plant Path., 49(1), 17-19.
[13] Gomez. K. A, Gomez A .A (1986). Statistical Procedure for Agricultural Research (2nd edn.). International Rice Research Institute, A Willey-Inter- Science, Publication pp. 28-192.
[14] Jadhav B.M., PeraneR.R., Kale A.A. and Pawar N.B., (2011), Morphological, pathological and molecular variability among Alternaria macrospora isolates causing leaf blight of cotton, Indian Phytopath. 64 (3): 254-257.
[15] Jha P, Kumar M, Meena PD and Lal H C, (2013), Dynamics and management of Alternaria blight disease of Indian mustard (Brassica juncea) in relation to weather parameters, J. of Oils. Bras., 4(2): 66-74.
[16] Kaur S, Singh G and Banga S S. ( 2007). Documenting variation in Alternaria brassicae isolates based on conidial morphology, fungicidal sensitivity and molecular profile. (in) Proceeding of the 12th International Rapeseed Congress, 26–30 March, Wuhan, China 4: pp 87–89.
[17] Khan M.M. (2011), Alternaria blight of mustard, a real farmer headache: morpho - physio variations and its cost effective management, lap lambert academic publishing. Isbn-13: 978-3845437187, pp-100.
[18] Kumar A, Katoch A, Sharma P, Kumari V and Kumar A ,(2014), Pathogenic and genetic variability in Alternaria brassicae infecting rapeseed-mustard and evaluation of resistance sources, Indian Phytopath. 67 (3) : 257-262.
[19] Kumar D, Maurya N, Bharati Y .K., Kumar A., Kumar K, Srivastava K, Chand G, Kushwaha C, Singh S.K,Mishra R. K and Kumar A,(2014), Alternaria blight of oilseed Brassicas: A comprehensive Review, Afri. J. of Microbiol. Res., 8 (30):2816-2829.
[20] Mathpal P., Punethav H., Tewari A.K. and Agrawal S., (2011), Biochemical defense mechanism in rapeseed-mustard genotypes against Alternaria blight disease, J. of Oils. Bras., 2 ( 2): 87-94.
[21] Meena P D, Gupta R, Rani A, Sharma P and Singh D, (2016), Effect of summer temperatures on survival of Alternaria brassicae in infected Indian mustard (Brassica juncea) debris and thermal death point variations amongst geographical isolates, J. Oils. Bras., 7 ( 1) : 45-51.
[22] Meena P.D, Awasthi R.P, Chattopadhyay. C, Kolte S.J and Arvind k, (2010), Alternaria blight: a chronic disease in rapeseed-mustard, J. of Oils. Bras., 1( 1), 1-11.
[23] Meena P.D, Gupta R, Sharma P, Rani A, Jha1 A .K, Meena H.S, Bala M, Singh D and Chowdappa P, (2016), Variability and growth response among Alternaria brassicae isolates causing black spot disease in oilseed Brassica, J. of Oils.Bras., 7 (2) : 126-138.
[24] Muthukumar A .and Venkatesh A, (2013), A new record of leaf blight of ribben plant caused by Alternaria alternata inIndia, J.on New Biol.Rep., 2(3): 228-230.
[25] Nikam P. S, Suryawanshi A. P and Chavan A.A, (2015), Pathogenic, cultural, morphological and molecular variabilityof eight isolates of Alternaria solani causing early blight of tomato, Afri. J. Of Biotech, 14(10): 872-877.
[26] Ramjegathesh R. and Ebenezar E.G. (2012). Morphological and Physiological Characters of Alternaria alternata Causing Leaf Blight Disease of Onion. Inter. J. of Plant Path. 3: 34-44.
[27] Saha, S., Garg, R., Venkataravanappa, Mishra V. P. K., Rai A. B. and Singh P. R.(2016). Molecular and Cultural Characterization of Alternaria brassicae Infecting Cauliflower in Uttar Pradesh, India. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 86: 485.
[28] Saharan GS, Naresh Mehta and Meena PD. (2016).Alternaria blight of crucifers: Biology, Ecology and Management. Springer Verlag, Singapore, pp 54, eBook ISBN 978-981-10-0021-8, Edition (1).
[29] Selvamani R., Pandian R.T.P. and Sharma P., (2014), Role of weather on Alternaria leaf spot development inCrucifers, Indian Phytopath. 67 (3) : 285-290.
[30] Sharma M, Deep S, Bhati D.S, Chowdappa. P, Selvamani. R and Sharma P.(2013). Morphological, cultural, pathogenic and molecular studies of Alternaria brassicae infecting cauliflower and mustard in India. Afri. J. of Biotec. 7( 26):3351-3363.
[31] Singh M, Singh H.K., Shiwangi, Maurya M, (2014), Morphological, Physiological and cultural variability in Alternaria brassicae isolates of Indian mustard, Brassicae juncea L. Czern & Coss. collected from different Agro climatic regions of India, Euro. J. of Biotec.y and Biosci., 3( 6): 33-37.
[32] Singh M.,. Singh H. K, Singh R. B., Shiwangi and Abhishek,(2015), Cultural and pathogenic variability in Alternaria brassicae isolates of Indian mustard [Brassica juncea (L.) Czern. and Coss] collected from different agro-climatic regions of India, Res. Environ. Life Sci.8(2) :281-286.
[33] Sofi T. A., Beig M A, Dar Gh H., Ahmad M, Hamid A, Ahangar F. A., Padder B. A. and Shah M. D.,(2013), Cultural, morphological, pathogenic and molecular characterization of Alternaria mali associated with Alternaria leaf blotch of apple, Afri. J. of Biotec. 12(4), 370-381.
[34] Soo-Sang H., Kwon Mi., Kim.B K, Han H K, and Nam Y G, (2016) Alternaria Leaf Spot Caused by Alternaria mali on Black Chokeberry in Korea, Res. Plant Dis. 22(1): 50-54.
[35] Tanya R. Marak, Ambesh .S.B and Srikanta. D. (2014). Cultural, Morphological and Biochemical Variations of Alternaria solani Causing Diseases on Solanaceous Crops, The Bioscan: 9(3): 1295-1300.
[36] Thakur P.R.(1999).Pathogen diversity and plant disease management. Indian Phytopath.52(1):1-9.
[37] Verma, P.R. and Saharan, G.S. (1994). Monograph on Alternaria diseases of crucifers. Saskatoon Research Centre Technical Bulletin 1994-6E, Agriculture and AgriFood Saskatoon.
[38] Yadav S.P, Kumar S, Prasad R, Upadhyay H and Bansal M, (2016), Identification of morphological, cultural and pathogenic variability of Alternaria brassicae causing Alternaria blight of Indian mustard (Brassic juncea), Indian Phytopath, 69( 1): 102-104.

Cite this Article: Show All (MLA | APA | Chicago | Harvard | IEEE | Bibtex)

Total View: 49 Downloads: 7 Page No: 2433-2442