Phenotypic Screening of Drought-Tolerant Lines for Brown Planthopper, Blast and Phytic Acid Content Assay of Rice (Oryza sativa L.)

Phenotypic Screening of Drought-Tolerant Lines for Brown Planthopper, Blast and Phytic Acid Content Assay of Rice (Oryza sativa L.) ( Vol-2,Issue-6,November - December 2017 )

Author: Pham Thi Thu Ha, Nguyen Thi Lang, Dang Minh Tam, PhamThi Kim Vang, Ramin Rayee

ijeab doi crossref DOI: 10.22161/ijeab/2.6.50

Keyword: Brown planthopper, blast, phytic acid content, screening, phenotype, drought-tolerant, rice

Abstract: Advanced drought tolerant lines were analysed for blast disease, brown planthopper (BPH), and phytic acid content. Thirsty lines of BC2F4 derived from OMCS2000/ IR75499-73-1 were used to screen for BPH and blast resistance. Three good resistant lines were screened against blast (45, 54, and 310) under greenhouse condition. As eight lines were identified to be resistant to BPH. The results further reveal that BC2F4-45 was the best line resistant to both BPH and blast disease. These lines will be useful in reducing grain phytic acid and improving the nutritional value of rice grain. Based on an assay for high phosphate germination stage of rice, the lowest content was found in the I5 variety (line 45). Hence, this line provides the urgent objective for breeders in cultivars of these crops to genetically enhance a healthy and functional diet. These characters will then need to be incorporated into high yield under drought stress with others such as disease and insect resistance.


[1] M. Hossain. Agricultural policies in Bangladesh: Evolution and impact on crop prodution. In state, market and Development: Essays in hornor of Rehman Sobhan, ed, A.A. Abdullan and A.R. Khan. Dhaka: University Press Litmited, 1996.
[2] N. Mishra, N.C. Hazarika, K. Narain and J. Mahanta. Nutritive value of non-mulberry and mulberry silkworm pupae and consumption pattern in Assam, India. Nutr. Res. 23 (10),2003, 1303–1311.
[3] R. Wassmann, S.V.K. Jagadish, S.Heuer, A.Ismail, E. Redona, R.Serraj, R.K. Singh, G.Howell, H.Pathak, and K. Sumfleth. Climate change affecting rice production: the physiological and agronomic basis for possible adaptation strategies. Advances in Agronomy. 101, 2009, 59–122.
[4] B. Bate, Z.W. Kundzedwicz, S. Wu and J. Paulutikof, eds. Climate change and water: IPCC Technical paper VI. Geneva, Seizerland: IPCC Secretariat, 2008.
[5] L.B. Jiang, K.F.Jao, D.J. Wangand J.C. Wu. Effects of different treatment methods of fungicide jinggangmycin on reproduction and vitellogenin gene (N1vg) expression into the brown planthopper Nilaparvatalugens (Stål) Hemiptera: Delphacididae. Pesticide Biochem Physiol. 102, 2012, 51–55.
[6] P.V. Du, R.C.Cabunagan, P.Q.Cabauatan, H.S. Choi, I.R. Choi, H.V. Chien, N.H. Huan. Yellowing syndrome of rice: Etiology, current status, and future challenges. Omonrice 15, 2007, 94–101.
[7] H.K. Manandhar, K. Shrestha and P.Amtya. Seed-borne diseases. In: Plant diseases, seed production and seed health testing in Nepal (S.B. Mathur, P.Amaty, K. Shrestha and H.K.Mananhar, eds). Danish Government, Institute of Seed Pathology for Developing Countries, Compenhagen, Denmark. 1992, 59–74.
[8] G.S. Khush and K.K. Jena. Current status and future prospects for research on blast resistance in rice (Oryza sativa L.). In: Wang GL, Valent B (eds) Advances in genetics, genomics and control of rice blast disease. Springer, Dordrecht.2009, pp 1–10.
[9] S.H. Hulbert, C.A. Web, S.M. Smithand Q. Sun. Resistance gene Complexs: Evolution and utilization. Annu Rev Phytopathol. 9, 2001, 285–312.
[10] J. Shi, H. Wang, J. Hazebroek, et al. The maize low-phytic acid 3 encodes a myo-inositol kinase that plays a role in phytic acid biosynthesis in developing seeds. The plant Journal. (42), 2005, 708–719.
[11] A. Beleia, L.T.T Thao and E.I. Ida. Lowering phytic phosphorus by hydration of soybean. Journal of Food Science. 58, 1993, 375–377.
[12] R. N. Trethewey and A. M. Smith. Starch mobilization in leaves. In: Advance in Photosynthesis (Eds): R.C. Legood, T.D. Shake, S. Von-Cammerer. Photosynthesis: Physiology and metabolism. Dordrecht, The Netherlands, Kulwer Academic Publishers. 9, 2000, 205–231.
[13] E. Loreti, P. Vernieri, A. Alpi and P. Perata. Repression of α-amylase activity by anoxia in grains of barley is independent of ethanol toxicity or action of abscisic acid. Plant Biology. 4, 2002, 266–272.
[14] P.T. T Ha, D.T. Khang, P.T.Tuyen, T.B. Toan, N.N. Huong, N.T. Lang, B.C. Buu, T.D. Xuan. 2016. Development of new drought tolerant breeding lines for Vietnam using marker-assisted backcrossing. International Letters of Natural Sciences. Vol. 59, 2016, 1–13.
[15] IRRI. Standard Evaluation System for Rice. IRRI, Manila, Philippines.1996.
[16] P. S. Chen, T.Y. Toribara and H. Warner. Microdeteminations of phosphorous. Analytical Chemical. 28, 1956, 1756–1758.
[17] N. Hayashi and Y. Fukuta. Proposal for a new international system of differentiating races of blast (PyriculariapryxaeCavara) using LTH monogenic lines in rice (Oryza sativa L.) JIRCAS working report No. 63. Tsukuba city, Ibaraki prefecture Japan, Japan International Research Center for Agricultural Science. 11–5, 2009.
[18] A. Sai Harini, S. Sai Kumar, B. Padma, S. Richa, D.M.Ayyappa and S. Vinay. Evaluation of rice genotypes for brown planthopper (BPH) resistance using molecular markers and phenotypic methods. Africa journal of biotechnology. 12 (19), 2013, 2525–2525.
[19] P.S. Sarao. Integrated Management of insect-pests of rice and basmati. Prog Farm, 51, 2015, 9–12.
[20] K. Gorman, Z. Liu, I. Denholm, K.U. Bruggen, K.U and R.Nauen. Neonicotinoid resistance in rice brown planthopper, Nilaparvatalugens. Pest Manag Sci. 64, 2008, 1122–1125.
[21] M. Matsumura, H. Takeuchi, M. Satoh, S. Sanasa-Morimura, A. Otuka, T. Watanabe, D.V. Thanh. Current status of insecticide resistance in rice planthoppers. In: K.L. Heongand B. Hardy. Planthoppers: New Threats to the Sustainability of Intensive Rice Production Systems in Asia. Los Baños, the Philippines: International Rice Research Institute.2009, 233–244.
[22] A.B. Khattak, A. Zeb and N. Bibi, et al. Influence of germination techniques on phytic acid and polyphenols content of chickpea (Cicerarietinum L.) sprouts. Food Chemistry. 104, 2007, 1074–1079.

Cite this Article: Show All (MLA | APA | Chicago | Harvard | IEEE | Bibtex)

Total View: 28 Downloads: 8 Page No: 3160-3165