Potential Impact of Salt Stress on Male Reproductive Development of Glycine Max (L.) Merr. (Soybean)

Potential Impact of Salt Stress on Male Reproductive Development of Glycine Max (L.) Merr. (Soybean) ( Vol-3,Issue-1,January - February 2018 )

Author: Semra Kilic, Arzu Seker

ijeab doi crossref DOI: 10.22161/ijeab/3.1.17

Keyword: crop, flowering, pollen germination, pollen tube growth, pollen viability.

Abstract: Product yield and the continuity of the quality of products of plants are in parallel with their ability to tolerate or adapt to environmental factors. For this reason, it is extremely important to determine the changes in the plants under various stress conditions. Male reproductive structures are directly related to product yield and quality, and they’re very sensitive to abiotic stress. Stress causes irreversible damage to plants depending on its amount and duration. The aim of this study is to determine the sensitivity of male reproductive structures of soybean seedlings and the critical salt concentration at which fertile pollen grains could be obtained in our soils whose salinity is increasing day by day. The selected soybean seedlings were exposed to increasing salt concentrations (50, 100, 150, 200, 250mM) for 6 months and they were compared with a control group in terms of flowering, pollen morphology (pollen size, exine and intine thickness, aperture structures), pollen viability, pollen germination, and pollen tube length. It was determined that, by affecting the growth process of soybean at varying grades, salt stress causes deformations in the plant’s reproductive structures and decreases it’s tolerance to salt stress.


[1] Flowers, T., 2006. PlantsandSalinity.Journal of ExperimentalBotany,57(5).
[2] Fahy, D.,Sanad, M.N., Duscha, K., Lyons, M., Liu, F., Bozhkov, P., 2017. Impact of salt stress, celldeath, andautophagy on peroxisomes: quantitative and morphological analyses usings mallfluorescent probe N-BODIPY. Scientific Research, 7:39069.
[3] Awasthi, R.,Kaushal, N., Vadez, V., Turner, N.C., Berger, J., Siddique, K.H.M., Nayyar, H.,2014. Individual and combined effects of transient drought and heat stress on carbon assimilation and seed filling in chickpea.FunctionalPlantBiology, 41:1148–1167.
[4] Dwivedi, S.L.,Sahrawat, K., Upadhyaya, H., Ortiz, R., 2013. Food, nutritionandagrobiodiversityunder global climatechange.Advances in Agronomy,120: 1-128.
[5] Pantuwan, G.,Fukai, S., Cooper, M., Rajatasereekul, S., O’toole, J.C., Basnayake, J., 2004. Yieldresponse of rice (OryzasativaL.) genotypes to drought under rainfed low lands: 4. Vegetativestagescreening in thedryseason.FieldCropsResearch, 89: 281–297.
[6] Arshad, M.S.,Farooq, M., Asch, F., Krishna, J.S., Prasad, P.V., Siddique, K.H., 2017. Thermal stress impacts reproductive development and grainyield in rice.Plant Physiology and Biochemistry, 11: 57-72.
[7] Prasad, P.V.,Bheemanahalli, R., Jagadish, S.K., 2017. Field crops and the fear of heatstress—Opportunities, challenges and future directions. Field Crops Research, 200: 114-121.
[8] Hoffmann, B., Munch, S., Schwägele, F., Neusüß, C., Jira, W., 2017. A sensitive HPLC-MS/MS screening method for the simultaneous detection of lupine, pea, and soy proteins in meatproducts.Food Control,71: 200-209.
[9] Essa, T.A., 2002. Effect of salinitystress on growth and nutrient composition of three soybean (Glycinemax L. Merrill) cultivars.Journal of Agronomy and CropScience,188(2): 86-93.
[10] Khan, M.S.A.,Chowdhury, J.A., Razzaque, M.A., Ali, M.Z., Paul, S.K., Aziz, M. A., 2017. Dry matter production and seed yield of soybean as affectedby post-flowering salinity and waterstress.Bangladesh Agronomy Journal, 19(2): 21-27.
[11] Amirjani, M.R., 2010.Effect of salinity stress on growth, mineral composition, prolinecontent, antioxidantenzymes of soybean.American Journal of Plant Physiology,5(6): 350-360.
[12] He, Y.,Chen, Y., Yu, C.L., Lu, K.X., Jiang, Q.S., Fu, J.L., Wang, G.M., Jiang, D.A., 2016. Photosynthesisandyieldtraits in different soybean lines in responseto salt stress.Photosynthetica,54(4): 630-635.
[13] Guo, C.,Ge, X.,Ma, H.,2013.The rice OsDIL gene plays a role in drought tolerance at vegetative and reproductive stages. Plant Molecular Biology,82:239–253.
[14] Guo, C.,Yao, L., You, C., Wang, S., Cui, J., Ge, X., Ma, H., 2016. MID1 plays an important role in response to drought stress during reproductive development. Plant Journal, 88(2): 280-293.
[15] Prasad, P.V.,Djanaguiraman, M., 2014. Response of floretfertilityandindividualgrainweight of wheat to high temperature stress: sensitive stages and thresholds for temperature and duration. Functional Plant Biology, 41(12): 1261-1269.
[16] Wodehouse, R.P., 1935. Pollengrains, McGraw-Hill, New York.
[17] Chhun, T., Aya, K., Asano, K., Yamamoto, E., Morinaka, Y., Watanabe, M., Kitano, H., Ashikari, M., Matsuoka, M., Ueguchi-Tanaka, M., 2007. Gibberellinre gulatespollenviability and pollentube growth in rice.Plant Cell, 19: 3876–3888.
[18] Song, J., Nada, K., Tachibana, S., 1999. Ameliorativeeffect of polyamines on thehightemperatureinhibition of in vitropollengerm. into mato.ScientiaHorticulturae, 80: 203-212.
[19] Kakani, V.G.,Reddy, K.R., Koti, S., Wallace, T.P., Prasad, P.V., Reddy, V.R., Zhao, D., 2005. Differences in in vitropollengermination and pollentube growth of cottoncultivars in responsetohightemperature.Annals of Botany, 96(1): 59-67.
[20] Luza, J.G.,Polito, V.S.,Weinbaum, S.A.,1987. Staminate bloom date and temperature responses of pollen germination and tube growth in two walnut (Juglans) species.AmericanJournal of Botany, 74:1898–1903.
[21] Dai, Q.J., Peng, S.B., Chavez, A.Q., Vergara, B.S.,1994. Intraspecific responses of 188 rice cultivars to enhanced UV-B radiation.Environmental and Experimental Botany, 34:422–433.
[22] Bhandari, K.,Siddique, K.H., Turner, N.C., Kaur, J., Singh, S., Agrawal, S.K., Nayyar, H., 2016. Heatstress at reproductive stage disrupts leaf carbohydrate metabolism, impairs reproductive function, and severely reduces seed yield in Lentil.Journal of Crop Improvement, 30(2): 118-151.
[23] Allu, A.D., Soja, A.M., Wu, A., Szymanski, J., Balazadeh, S.,2014. Salt stress and senescence: identification of cross-talk regulatory components.Journal of Experimental Botany,65:3993–4008.
[24] Ghanem, M.E., Van,Elteren, J., Albacete, A., Quinet, M., Martinez-Andújar, C., Kinet, J.M., 2009. Impact of salinity on early reproductive physiology of tomato (Solanumlycopersicum) in relationto a heterogeneous distribution of toxicions in flowerorgans.FunctionalPlantBiology,36(2): 125-136.
[25] Pushpavalli, R.,Quealy, J., Colmer, T.D., Turner, N.C., Siddique, K.H.M., Rao, M.V., Vadez, V., 2016. Salt stress delayed flowering and reduced reproductive success of chickpea (Cicerarietinum L.), a response associated with Na+ accumulation in leaves. Journal of Agronomy and Crop Science, 202(2): 125-138.
[26] Torabi, F.,Ahmad, M.A.J.D., Enteshari, S., Irian, S., Nabiuni, M., 2013. Effects of salinity on thedevelopment of hydroponically grown borage (Boragoofficinalis L.) malegametophyte.Notulae Botanicae Horti Agrobotanici, 41(1): 65.
[27] Roy, S.J.,Negrao, S., Tester, M., 2014. Salt resistant crop plants.CurrentOpinion in Biotechnology, 26: 115–124.
[28] Mccallum, B.,Chang, S.M., 2016. Pollencompetition in style: Effects of pollen size on siringsuccess in the hermaphroditic common morning glory, Ipomoeapurpurea. American Journal of Botany, 103(3): 460-470.
[29] He, H.,Serraj, R., 2012. Involvement of peduncleelongation, antherdehiscence and spikeletsterility in uplandriceresponsetore productive-stage drought stress. Environmental and Experimental Botany, 75: 120–127.
[30] Matamoro‐Vidal, A.,Raquin, C., Brisset, F., Colas, H., Izac, B., Albert, B., Gouyon, P.H., 2016. Links betweenmorphologyandfunction of thepollenwall: an experimental approach. Botanical Journal of theLinneanSociety,180(4): 478-490.
[31] Prieu, C.,Matamoro-Vidal, A., Raquin, C., Dobritsa, A., Mercier, R., Gouyon, P.H., Albert, B., 2016. Aperturenumber influences pollen survival in Arabidopsismutants.American Journal of Botany, 103(3): 452-459.
[32] Albert, B.,Raquin, C., Prigent, M., Nadot, S., Brisset, F., Yang, M., Ressayre, A., 2011. Successive micro sporogenesis affects pollenaperture pattern in the tam mutant of Arabidopsisthaliana. Annals of Botany,107(8): 1421-1426.
[33] Storme, N.,Geelen, D., 2014. Theimpact of environmentalstress on malere productive development in plants: biological processes and molecular mechanisms. Plant, Cell and Environment, 37(1): 1-18.
[34] Thakur, P.,Kumar, S., Malik, J.A., Berger, J.D.,Nayyar, H.,2010.Cold stress effects on reproductive development in grain crops: an overview. Environmental and Experimental Botany, 67:429–443.
[35] Müller, F.,Rieu, I., 2016. Acclimation to high temperature during pollen development. Plant Reproduction,29(1-2): 107-118.
[36] Dwivedi, S.K.,Basu, S., Kumar, S., Kumar, G., Prakash, V., Kumar, S., 2017. Heat stress induced impairment of starch mobilisation regulates pollen viability and grain yield in wheat: Study in EasternIndo-Gangetic Plains.Field Crops Research, 206: 106-114.
[37] Novara, C., Ascari, L., La,Morgia, V., Reale, L., Genre, A., Siniscalco, C., 2017. Viability and germinability in long term storage of Corylusavellanapollen. Scientia Horticulturae, 214: 295-303.
[38] Baby, T.,Collins, C., Tyerman, S.D., Gilliham, M., 2016. Salinitynegativelyaffectspollentube growth and fruit set in grapevines and cannot be amelioratedby silicon.American Journal of Enology and Viticulture, 2015.15004.
[39] Monihan, S.M.,Magness, C.A., Yadegari, R., Smith, S.E., Schumaker, K.S., 2016. Arabidopsis CALCINEURIN B-LIKE10 functionsindependently of the sos pathway during reproductive development in salineconditions. Plant Physiology, 174(4): 334.

Cite this Article: Show All (MLA | APA | Chicago | Harvard | IEEE | Bibtex)

Total View: 33 Downloads: 18 Page No: 132-139