A Novel UV-Vis Spectrophotometric Method for Quantifying Rifaximin: Method Development and Validation

DOI: 10.22161/ijeab.94.7

Abstract:
This study evaluated the standardization and method validation of rifaximin using different acid and phosphate buffer conditions (pH 1.2, 6.8 and 7.4). Although all tested conditions showed excellent precision (less than 2% RSD), limitations in linearity and precision were observed. Although the correlation coefficients were high (0.9898 to 0.9972), they deviated from the ideal (1.0), indicating possible nonlinearities. Accuracy ranged from 89.45% to 94.16%, indicating slight under- or overestimation of rifaximin concentration. These limitations compromise the reliability of the rifaximin quantification method. Other optimization strategies are recommended, including exploring different pH conditions, refining the concentration range of the standard curve, and considering alternative analytical methods such as HPLC when possible. By addressing these limitations, a more robust and reliable method for rifaximin standardization can be achieved.

Keywords:
Rifaximin, Spectrophotometer, Standardization and method validation, Robustness, RSD

Article Info:
Received: 01 Jun 2024; Received in revised form: 03 Jul 2024; Accepted: 11 Jul 2024; Available online: 19 Jul 2024

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