Addition of Different Nitrogen Sources on the Cocoa Pod (Theobroma Cacao) fermented with Pleurotus Ostreatus

Author: Junaidah, Nuraini, Ade djulardi

ijeab doi crossref DOI: 10.22161/ijeab.45.6

Keyword: Fermentation, Cocoa pod, Nitrogen source, Pleurotus osteratus

Abstract: This research was aimed the composition of the substrate, inoculum dose and fermentation time suitable for the growth of Pleurotus ostreatus on cocoa pod substrate mixtures with different nitrogen sources (tofu waste, soy milk waste and rice bran) of the cellulase enzyme activity, laccase enzyme activity, crude fiber decreased, and crude fiber digestibility. This study used an experimental method with a completely randomized design (RAL) 3x3x3 factorial with 2 replications. Factor A is a substrate composition with a ratio of 80% cocoa pod and 20% for tofu waste, soy milk waste, and rice bran. Factor B is an inoculum dose of 6%, 8% and 10% with Factor C is the fermentation time of 7, 9 and 11 days. The results of the analysis showed that an interaction of the substrate composition and fermentation time with Pleurotus ostreatus which had a significant effect (P <0.05) of the activity of cellulase enzymes, laccase enzyme activity, crude fiber decreased and crude fiber digestion. The results of the study can be concluded that the selected treatment was found in the composition of 80% cocoa pod and 20% tofu waste with 9 days fermentation time (A1C2). In this condition cellulase enzyme activity was obtained of 2.70 U/ml, the laccase enzyme activity of 12.33 U/ml, crude fiber decreased of 44.01% and crude fiber digestibility of 53.78%.

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Cite this Article:

MLA

Junaidah, Nuraini, Ade djulardi et al."Addition of Different Nitrogen Sources on the Cocoa Pod (Theobroma Cacao) fermented with Pleurotus Ostreatus ". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878),vol 4, no. 5, 2019, pp.1334-1340 AI Publications doi:10.22161/ijeab.45.6

APA

Junaidah, Nuraini, Ade djulardi, P.(2019).Addition of Different Nitrogen Sources on the Cocoa Pod (Theobroma Cacao) fermented with Pleurotus Ostreatus . International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).4(5), 1334-1340.10.22161/ijeab.45.6

Chicago

Junaidah, Nuraini, Ade djulardi, P.(2019).Addition of Different Nitrogen Sources on the Cocoa Pod (Theobroma Cacao) fermented with Pleurotus Ostreatus . International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).4(5), pp.1334-1340.

Harvard

Junaidah, Nuraini, Ade djulardi. 2019."Addition of Different Nitrogen Sources on the Cocoa Pod (Theobroma Cacao) fermented with Pleurotus Ostreatus ". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).4(5):1334-1340.Doi:10.22161/ijeab.45.6

IEEE

Junaidah, Nuraini, Ade djulardi."Addition of Different Nitrogen Sources on the Cocoa Pod (Theobroma Cacao) fermented with Pleurotus Ostreatus ", International Journal of Environment Agriculture and Biotechnology,vol.4,no. 5, pp.1334-1340,2019.

Bibtex

@article { junaidah2019addition,
title={Addition of Different Nitrogen Sources on the Cocoa Pod (Theobroma Cacao) fermented with Pleurotus Ostreatus },
author={Junaidah, Nuraini, Ade djulardi , R},
journal={International Journal of Environment Agriculture and Biotechnology},
volume={4},
year= {2019} ,
}