Antibacterial and antibiofilm activities of quercetin against clinical isolates of Staphyloccocus aureus and Staphylococcus saprophyticus with resistance profile

DOI: 10.22161/ijeab/3.5.50

Keyword: Resistance; biofilm; quercetin; antibacterial activity; antibiofilm activity.

Abstract: The aim of this study was to determine the antibacterial and antibiofilm properties of quercetin against clinical isolates of Staphyloccocus aureus and Staphylococcus saprophyticus with resistance profile. The antibacterial activity of quercetin was performed by the determination of the minimum inhibitory concentration (MIC) through the microdilution method according to the Clinical and Laboratory Standards Institute (CLSI). The percentage of inhibition of Staphylococcus spp. biofilm, after treatment with sub-inhibitory concentrations of quercetin (MIC/2 and MIC/4), was evaluated by the violet crystal assay. Quercetin showed an antimicrobial activity against clinical isolates of methicillin-susceptible S. aureus (MSSA) (MIC = 250 µg/ml), methicillin-resistant S. aureus (MRSA) (MIC = 500 µg/ml), vancomycin-intermediate S. aureus (VISA) (MIC = 125 and 150 µg/ml), S. saprophyticus resistant to oxacillin (MIC = 62.5 to 125 µg/ml), vancomycin-resistant S. aureus (VRSA) and S. saprophyticus resistant to oxacillin and vancomycin (MIC = 500 to 1000 µg/ml). At MIC/2 and MIC/4 the quercetin inhibit 46.5 ± 2.7% and 39.4 ± 4.3% of the S. aureus biofilm, respectively, and 51.7 ± 5.5% and 46.9 ± 5.5% of the S. saprophyticus biofilm, respectively. According to the results of this study, it was noticed that the quercetin presented an antibacterial activity against strains of Staphylococcus spp. with resistance profile and also inhibited the bacterial biofilm production even in sub-inhibitory concentrations.

Total View: 3017

Downloads: 702

Page No: 1948-1958

Cite this Article:

Sérgio Dias da Costa Júnior, João Victor de Oliveira Santos, Luís André de Almeida Campos, Marcela Araújo Pereira, Nereide Stela Santos Magalhães, Isabella Macário Ferro Cavalcanti et al."Antibacterial and antibiofilm activities of quercetin against clinical isolates of Staphyloccocus aureus and Staphylococcus saprophyticus with resistance profile". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878),vol 3, no. 5, 2018, pp.1948-1958 AI Publications doi:10.22161/ijeab/3.5.50

Sérgio Dias da Costa Júnior, João Victor de Oliveira Santos, Luís André de Almeida Campos, Marcela Araújo Pereira, Nereide Stela Santos Magalhães, Isabella Macário Ferro Cavalcanti, P.(2018).Antibacterial and antibiofilm activities of quercetin against clinical isolates of Staphyloccocus aureus and Staphylococcus saprophyticus with resistance profile. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).3(5), 1948-1958.10.22161/ijeab/3.5.50

Sérgio Dias da Costa Júnior, João Victor de Oliveira Santos, Luís André de Almeida Campos, Marcela Araújo Pereira, Nereide Stela Santos Magalhães, Isabella Macário Ferro Cavalcanti, P.(2018).Antibacterial and antibiofilm activities of quercetin against clinical isolates of Staphyloccocus aureus and Staphylococcus saprophyticus with resistance profile. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).3(5), pp.1948-1958.

Sérgio Dias da Costa Júnior, João Victor de Oliveira Santos, Luís André de Almeida Campos, Marcela Araújo Pereira, Nereide Stela Santos Magalhães, Isabella Macário Ferro Cavalcanti. 2018."Antibacterial and antibiofilm activities of quercetin against clinical isolates of Staphyloccocus aureus and Staphylococcus saprophyticus with resistance profile". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).3(5):1948-1958.Doi:10.22161/ijeab/3.5.50

Sérgio Dias da Costa Júnior, João Victor de Oliveira Santos, Luís André de Almeida Campos, Marcela Araújo Pereira, Nereide Stela Santos Magalhães, Isabella Macário Ferro Cavalcanti."Antibacterial and antibiofilm activities of quercetin against clinical isolates of Staphyloccocus aureus and Staphylococcus saprophyticus with resistance profile", International Journal of Environment Agriculture and Biotechnology,vol.3,no. 5, pp.1948-1958,2018.

@article { sérgiodiasdacostajúnior2018antibacterial,
title={Antibacterial and antibiofilm activities of quercetin against clinical isolates of Staphyloccocus aureus and Staphylococcus saprophyticus with resistance profile},
author={Sérgio Dias da Costa Júnior, João Victor de Oliveira Santos, Luís André de Almeida Campos, Marcela Araújo Pereira, Nereide Stela Santos Magalhães, Isabella Macário Ferro Cavalcanti , R},
journal={International Journal of Environment Agriculture and Biotechnology},
volume={3},
year= {2018} ,
}