Comparative Analysis of Total Chlorophyll (TC) and Total Soluble Sugar (TSS) in Selected Species of the Poaceae and Brassicaceae Families

DOI: 10.22161/ijeab.104.39

Abstract:
Plant biochemical constituents play a crucial role in regulating photosynthesis, growth, metabolism, and overall crop productivity. Among these constituents, total chlorophyll (TC) and total soluble sugar (TSS) are widely recognized as important physiological indicators reflecting photosynthetic efficiency, carbon assimilation, and plant metabolic status. The present study aimed to comparatively evaluate the mean total chlorophyll and total soluble sugar content of selected species belonging to the Poaceae and Brassicaceae families using a literature-based comparative approach. A total of 40 representative species, comprising 20 species from Poaceae and 20 species from Brassicaceae, were included in the analysis. Published biochemical data were compiled from scientific literature, standardized, and comparatively analyzed to assess interspecific and interfamily variation. The analysis revealed considerable variability in both biochemical parameters among the selected species. Mean total chlorophyll content ranged from 1.40 to 3.50 mg g⁻¹ FW, while total soluble sugar varied between 4.00 and 19.00 °Brix. Within the Poaceae family, Bambusa vulgaris exhibited the highest mean total chlorophyll content (3.35 mg g⁻¹ FW), whereas Saccharum officinarum recorded the highest total soluble sugar (19.00 °Brix). Among the Brassicaceae species, Brassica oleracea var. acephala (kale) showed the highest mean total chlorophyll (3.50 mg g⁻¹ FW), while Armoracia rusticana (horseradish) exhibited the highest total soluble sugar (10.25 °Brix). Overall, Poaceae species demonstrated greater variation in soluble sugar accumulation, whereas Brassicaceae species, particularly leafy vegetables, generally exhibited comparatively higher chlorophyll concentrations. A positive association between total chlorophyll and total soluble sugar was observed in several species, suggesting that enhanced photosynthetic pigment concentration contributes to improved carbohydrate accumulation.The study demonstrates substantial biochemical diversity among economically important members of the Poaceae and Brassicaceae families and highlights the usefulness of total chlorophyll and total soluble sugar as physiological indicators for comparative evaluation. The findings provide baseline information that may support future studies on crop physiology, biochemical characterization, nutritional assessment, and breeding strategies aimed at improving photosynthetic efficiency and crop productivity.

Keywords:
Poaceae, Brassicaceae, total chlorophyll, total soluble sugar, comparative analysis, plant biochemistry, photosynthesis, crop physiology.

Article Info:
Received: 19 Jul 2025; Received in revised form: 15 Aug 2025; Accepted: 22 Aug 2025; Available online: 31 Aug 2025

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