D-Amino Acid Oxidase Production from Cassava Glucose Syrup by Trigonopsis variabilis

DOI: 10.22161/ijeab/3.3.27

Abstract:
Three alternative carbon sources (molasses, cassava glucose syrup and sorghum fructose syrup) on the production of D-amino acid oxidase (DAAO) from Trigonopsis variabilis (TvDAAO) has been studied.The aim of this study was to screen out the best alternative carbon sources that can be utilized in TvDAAO production, and to study the effect of the additional nitrogen sources. Screening of carbon sources were carried out using glucose, molasses, cassava glucose syrup (CGS) and sorghum fructose syrup (SFS), at the same glucose levels. Analysis of TvDAAO was measured by o-phenylenediamine/horseradish peroxidase coupling assay. The best alternative carbon source was selected for optimization at various concentrations. The enzyme characteristic was done by determining the stability of enzyme to temperature and pH, and the enzyme kinetic parameter was also observed. The screening showed that cassava sugar syrup is the best alternative carbon source. The optimum concentration of cassava glucose syrup is at 10% of glucose levels, which will produce TvDAAO with the activity equal to 166.8861 U/g yeast cell dry weight. The enzyme characteristics stable at 4-10°C and pH 8, with Vmax value was 0.007 μmol/minute and KM was 78 mM. The used of cassava glucose syrup does not require any additional nitrogen source and it is became the advantageous of CGS as an alternative carbon source in terms of efficiency and economical of TvDAAO production.

Keywords:
Cassava Glucose Syrup, D-Amino Acid Oxidase, Trigonopsis variabilis.

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