Determination of Physiochemical Properties and Lactic Acid Bacteria Presence in Ackee (Blighia sapida) Fruit

DOI: 10.22161/ijeab/3.3.47

Abstract:
This study covers proximate analysis and Antimicrobial activities of cell free extract of lactic acid bacterial from Blighia sapida fruit (Ackee). The proximate analysis of Blighia sapida were determined using standard methods which varied in contents. The proximate analysis of the sample were ash content (6.10±0.03%), moisture content (5.43±0.12%), fat content (14.60±0.02%), crude fibre content (25.12±0.02%), protein content (14.19±0.09%) and carbohydrate content (34.19±0.23%). Probiotic of lactic acid bacteria isolated from Blighia sapida (Ackee) were identified as Lactobacillus leshmanni, and Lactobacillus acidophilus, with Streptomycin sulphate as control. Antimicrobial activities of the lactic acid isolated were determined by the agar well diffusion methods against pathogenic bacteria: Escherichia coli, Staphylococcus aureus, Xanthomonas sp, Pseudomonas aeruginosa, Salmonella typhi and Bacillus subtilis. The zone of inhibition ranged from 3mm to 25mm and control ranged from 23mm to 30mm. Lactobacillus acidophilus displayed high antimicrobial activity compared with Lactobacillus leshmanni showing its probiotic potential of lactic acid bacteria use for food supplement.

Keywords:
Blighia sapida, probiotic, L. leshmanni, L. acidophilus, proximate analysis.

Cite this Article: