Effect of differential expression of pro-region on the transglutaminase productivity in pichia pastoris

DOI: 10.22161/ijeab.61.35

Keyword: Pichia pastoris, protein folding, pro-peptide, transglutaminase.

Abstract: Transglutaminase (TG) induces protein cross-linking by catalyzing the reaction of acyl transfer. Mature sequence of TG is inactive when express without its pro-region. Since pro-region is critical for inhibiting the TG’s action and for correctly folding it extracellularly, the production is either poor or forming inclusion bodies without its pro-region. One of the fundamental steps for higher yield to date is to increase the transcriptional level of the recombinant gene by generating multicopies that could only be accomplished by cloning the concatemers. Here, co-expressing strain was successfully generated by incorporating pro-region into ribosomal DNA (rDNA) sites to achieve different copies. The maximum enzyme activity was up to 3.9u/ml compared to the wild type that was only 2.1u/ml in shake flasks being inducted for 96hrs.This research work provides an important strategy for its pro-region to take advantage of the degree of transglutaminase folding.

Article Info: Received: 03 Dec 2020; Received in revised form: 24 Jan 2021; Accepted: 13 Feb 2021; Available online: 28 Feb 2021

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Page No: 282-289

Cite this Article:

Aqeel Sahibzada Muhammad, Al-Adeeb Abdulqader, Waleed AL-Ansi, Sharoon Ejaz, Lu Xia, Song Liu et al."Effect of differential expression of pro-region on the transglutaminase productivity in pichia pastoris". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878),vol 6, no. 1, 2021, pp.282-289 AI Publications doi:10.22161/ijeab.61.35

Aqeel Sahibzada Muhammad, Al-Adeeb Abdulqader, Waleed AL-Ansi, Sharoon Ejaz, Lu Xia, Song Liu, P.(2021).Effect of differential expression of pro-region on the transglutaminase productivity in pichia pastoris. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).6(1), 282-289.10.22161/ijeab.61.35

Aqeel Sahibzada Muhammad, Al-Adeeb Abdulqader, Waleed AL-Ansi, Sharoon Ejaz, Lu Xia, Song Liu, P.(2021).Effect of differential expression of pro-region on the transglutaminase productivity in pichia pastoris. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).6(1), pp.282-289.

Aqeel Sahibzada Muhammad, Al-Adeeb Abdulqader, Waleed AL-Ansi, Sharoon Ejaz, Lu Xia, Song Liu. 2021."Effect of differential expression of pro-region on the transglutaminase productivity in pichia pastoris". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).6(1):282-289.Doi:10.22161/ijeab.61.35

Aqeel Sahibzada Muhammad, Al-Adeeb Abdulqader, Waleed AL-Ansi, Sharoon Ejaz, Lu Xia, Song Liu."Effect of differential expression of pro-region on the transglutaminase productivity in pichia pastoris", International Journal of Environment Agriculture and Biotechnology,vol.6,no. 1, pp.282-289,2021.

@article { aqeelsahibzadamuhammad2021effect,
title={Effect of differential expression of pro-region on the transglutaminase productivity in pichia pastoris},
author={Aqeel Sahibzada Muhammad, Al-Adeeb Abdulqader, Waleed AL-Ansi, Sharoon Ejaz, Lu Xia, Song Liu , R},
journal={International Journal of Environment Agriculture and Biotechnology},
volume={6},
year= {2021} ,
}