Histochemical Observations of Eleusine coracana Stem Tissues Under Multiple Abiotic Stresses

DOI: 10.22161/ijeab.113.9

Abstract:
This study investigates the histochemical changes of lignin deposition in the stems of seven finger millet genotypes under Drought, Salinity, Heat, Cold, and Heavy metal stress using the Phloroglucinol-HCl staining method. Well-grown plants were subjected to individual stresses at the pre-reproductive stage, and stem cross-sections were analyzed for lignin accumulation and structural differences under light microscope. This study revealed significant genotypic variation among genotypes and also in lignin deposition under stress conditions compared to respective controls. Among different stresses given, heat is showing maximum effect on vascular bundles and stem structural stability of plant. Based on lignin, stress tolerant and susceptible varieties were identified. Results suggested few genotypes which can be used as animal feed, fodder, are agronomically important and industrially applicable genotypes.

Keywords:
Abiotic stress, Finger millet (Eleusine coracana), Lignin, Vascular tissue.

Article Info:
Received: 25 Apr 2026; Received in revised form: 22 May 2026; Accepted: 27 May 2026; Available online: 04 Jun 2026

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