In Vitro Study on total Phenols, Flavonoids Content and DPPH Activity of Withania Species

Author: Nishtha Raj, Ayushi Agarwal, Neelam Chaturvedi

ijeab doi crossref DOI: 10.22161/ijeab/2.2.49

Keyword: DPPH (2, 2-diphenyl-1-picrylhydrazyl), Total Flavonoids Content, Total Phenol Content, Withania somnifera, Withania coagulans.

Abstract: The escalating interest in appraisal of antioxidant power of herbal plant as medicine, the current study was carried out to explore the antioxidant potential of aqueous extracts of Withania somnifera root and Withania coagulan fruit in-vitro. Antioxidant activity; total phenol,total flavonoids and DPPH free radical scavenging assay of Withania somnifera root and Withania coagulans fruit aqueous extracts were determined by using reference standards gallic acid, quercetin and ascorbic acid, respectively. The highest total phenols content (mgGAE/g) and total flavonoids content (mgQE/g) was found to be 33.1±0.82 and 1.86±0.01 respectively in aqueous somnifera root extracts as compared to coagulans fruit extract . The DPPH radical scavenging activity of the both extracts was increased with the increasing concentration and was observed high in aqueous extract insomniferaroot (IC50= 54) than coagulans fruit (69μg/ml) aqueous extract.Thus,Withania somnifera root has potent antioxidant activity and may serve as a good pharmacotherapeutic agent which could be explored to provide affordable medicines to masses.

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Cite this Article:

MLA

Nishtha Raj, Ayushi Agarwal, Neelam Chaturvedi et al."In Vitro Study on total Phenols, Flavonoids Content and DPPH Activity of Withania Species". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878),vol 2, no. 2, 2017, pp.0946-0950 AI Publications doi:10.22161/ijeab/2.2.49

APA

Nishtha Raj, Ayushi Agarwal, Neelam Chaturvedi, P.(2017).In Vitro Study on total Phenols, Flavonoids Content and DPPH Activity of Withania Species. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).2(2), 0946-0950.10.22161/ijeab/2.2.49

Chicago

Nishtha Raj, Ayushi Agarwal, Neelam Chaturvedi, P.(2017).In Vitro Study on total Phenols, Flavonoids Content and DPPH Activity of Withania Species. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).2(2), pp.0946-0950.

Harvard

Nishtha Raj, Ayushi Agarwal, Neelam Chaturvedi. 2017."In Vitro Study on total Phenols, Flavonoids Content and DPPH Activity of Withania Species". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).2(2):0946-0950.Doi:10.22161/ijeab/2.2.49

IEEE

Nishtha Raj, Ayushi Agarwal, Neelam Chaturvedi."In Vitro Study on total Phenols, Flavonoids Content and DPPH Activity of Withania Species", International Journal of Environment Agriculture and Biotechnology,vol.2,no. 2, pp.0946-0950,2017.

Bibtex

@article { nishtharaj2017in,
title={In Vitro Study on total Phenols, Flavonoids Content and DPPH Activity of Withania Species},
author={Nishtha Raj, Ayushi Agarwal, Neelam Chaturvedi , R},
journal={International Journal of Environment Agriculture and Biotechnology},
volume={2},
year= {2017} ,
}