Influence of Plant Growth Regulators on Somatic Embryogenesis Induction in Seriphidium herba-album

Author: Hemaid I. A. Soliman, Fatma M. Abo-El-Hasan, Ayman S. El-seedy, Yasser M. Mabrouk

ijeab doi crossref DOI: 10.22161/ijeab/3.4.17

Keyword: Seriphidium herba-album, callus induction, somatic embryos, plant growth regulators (PGRs).

Abstract: Seriphidium herba-album (syn. Artemisia herba-alba) is a medicinal, aromatic, greenish-silver herb. It is used widely in folk medicine for treatment of diarrhea, abdominal cramps and in the healing of external wounds. It's also used for the treatment of diabetes mellitus, neurological disorders as epilepsy, Alzheimer’s disease, depression and jaundice. In this study we assessed the protocol for callus induction, maturation of somatic embryogenesis, frequency of germination and conversion into plantlets for leaf explants of Seriphidium herba-album using different concentrations of PGRs. Highest induction frequencies of embryogenic calli occurred after 35 days on MS medium supplemented with 1.5 mg L-1 2,4-D and 0.5 mg L-1 BAP. Optimum MS medium for higher frequency of matured somatic embryos was recorded using 5.0 mg L-1 BAP and 0.5 mg L-1 NAA and somatic embryos also induced young in vitro grown plantlets when cultured in the medium containing GA3 and kinetin. Hence, attempts to induce direct somatic embryogenesis have been achieved up to embryo regeneration and maturation.

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Cite this Article:

MLA

Hemaid I. A. Soliman, Fatma M. Abo-El-Hasan, Ayman S. El-seedy, Yasser M. Mabrouk et al."Influence of Plant Growth Regulators on Somatic Embryogenesis Induction in Seriphidium herba-album". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878),vol 3, no. 4, 2018, pp.1264-1270 AI Publications doi:10.22161/ijeab/3.4.17

APA

Hemaid I. A. Soliman, Fatma M. Abo-El-Hasan, Ayman S. El-seedy, Yasser M. Mabrouk, P.(2018).Influence of Plant Growth Regulators on Somatic Embryogenesis Induction in Seriphidium herba-album. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).3(4), 1264-1270.10.22161/ijeab/3.4.17

Chicago

Hemaid I. A. Soliman, Fatma M. Abo-El-Hasan, Ayman S. El-seedy, Yasser M. Mabrouk, P.(2018).Influence of Plant Growth Regulators on Somatic Embryogenesis Induction in Seriphidium herba-album. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).3(4), pp.1264-1270.

Harvard

Hemaid I. A. Soliman, Fatma M. Abo-El-Hasan, Ayman S. El-seedy, Yasser M. Mabrouk. 2018."Influence of Plant Growth Regulators on Somatic Embryogenesis Induction in Seriphidium herba-album". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).3(4):1264-1270.Doi:10.22161/ijeab/3.4.17

IEEE

Hemaid I. A. Soliman, Fatma M. Abo-El-Hasan, Ayman S. El-seedy, Yasser M. Mabrouk."Influence of Plant Growth Regulators on Somatic Embryogenesis Induction in Seriphidium herba-album", International Journal of Environment Agriculture and Biotechnology,vol.3,no. 4, pp.1264-1270,2018.

Bibtex

@article { hemaidi.a.soliman2018influence,
title={Influence of Plant Growth Regulators on Somatic Embryogenesis Induction in Seriphidium herba-album},
author={Hemaid I. A. Soliman, Fatma M. Abo-El-Hasan, Ayman S. El-seedy, Yasser M. Mabrouk , R},
journal={International Journal of Environment Agriculture and Biotechnology},
volume={3},
year= {2018} ,
}