Pathogenic and Molecular Characterization of Fusarium oxysporum f.sp. ciceri Causing Chickpea Wilt through ISSR Markers

DOI: 10.22161/ijeab/1.2.1

Abstract:
In the present investigation the pathogenic and genetic variability was assayed, amongst the seven isolates of Fusarium oxysporum f.sp. ciceri (Foc) collected from different agro-climatic zones of Maharashtra State, India. The isolates of Fusarium oxysporum f.sp.ciceri were confirmed by SCAR marker which yielded 1.5 KD band. The pathogenicity of each isolate was confirmed using the wilt susceptible chickpea genotype JG-62. On the basis of pathogenic ability the isolates were grouped as highly pathogenic (FOC-2, FOC-5, FOC-6), strongly pathogenic (FOC-1,FOC-3) and moderately pathogenic (FOC-4,FOC-7). Eight Inter Simple Sequence Repeats primers (ISSRs) were used to determine the genetic variability in seven isolates Fusarium oxysporum f.sp. ciceri. The seven primers produced 80 scorable bands. Off 80 bands, 73 bands were polymorphic and average level of polymorphism was 91.25 per cent. In UPGMA analysis, Foc-1 (Wardha) was found to have higher value of similarity coefficient (0.8375) whereas Foc-2 (Lonar) was found to have lower value of similarity coefficient (0.4625). The isolates of Fusarium oxysporum f.sp.ciceri were grouped into two major clusters. First group, cluster-A includes isolates belonging to Wardha, Washim and Lonar. Second group, cluster-B includes Nashik, Ahmednagar, Rahuri and Pune. It shows that Foc-1 (Wardha) have higher value of similarity coefficient with Foc-3 (Washim) whereas Foc-2 (Lonar) have lower value of similarity coefficient with Foc-5 (Ahmednagar).The similarity matrix indicated that seven isolates of Fusarium oxysporum f.sp.ciceri exhibited in between 46-84 per cent similarity coefficient.

Keywords:
Fusarium oxysporum f.sp. ciceri, ISSR Markers, Pathogenic variation.

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