Pathological, Bio-chemical and Molecular diversity amongst the isolates of Xanthomonas axonopodis pv. citri causing Citrus canker in acid lime from different agro-climatic region of India

DOI: 10.22161/ijeab/1.2.25

Keyword: Biochemical test , ERIC, Detached leaf assay, REP, Xanthomonas axonopodis pv. Citri.

Abstract: In present investigation isolates of Xanthomonas axonopodis pv. citri (Xac)causing citrus canker were collected from fourteen agro climatic regions of India. The pathogenic variability of Xac was studied on four different varieties of acid lime viz. Sai sarbati, Phule sarbati, Pramalini and PKM-1 by using detached leaf assay. The isolates showed varied reaction in the symptoms development. The isolates viz. Xac- III, Xac- V, Xac- VII, Xac- XI, Xac- XIII and Xac- XIV found highly virulent and showed of typical symptoms at the point of inoculation within 7 to 9 days. The isolates Xac- I, Xac- II and Xac- IV were found less virulent, developed symptoms after 13 to 16 days of inoculation. The isolates Xac- I and Xac- II failed to develop symptom on variety PKM-1. Further all 15 selected isolates were subjected to biochemical characterization; all isolates were found rod shaped, gram –ve, with colony colour ranging from pale yellow to dark yellow. The isolate were positive for Catalase, KOH and H2S production, hydrolyse starch and gelatin liquefaction. All isolates produce acid from Trahalose. Whereas all isolates fails to produce Indol. The Random Amplified Polymorphic DNA (RAPD) was used to study the variation amongst the 15 isolates of Xac. A total of 27 RAPD primers were screened. Off which 19 primers showed amplification and produced scorable bands with high degree of polymorphism. A total 220 amplicons were obtained of which 218 amplicons were polymorphic with 99.52% level of polymorphism. The banding profile varied from minimum 5 band types (OPB- 1) to maximum 21 band type (REP) indicating the high molecular variability amongst all the fifteen isolates of Xac.The similarity coefficient ranged from 0.27 to 0.68. The maximum genetic similarity was found amongst the isolate from Uttar Pradesh (Xac- V) and Shriganganagar (Xac- XIV) i.e. 0.68

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Madhuri Katkar, K.S.Raghuwanshi, V. P. Chimote, S.G.Borkar et al."Pathological, Bio-chemical and Molecular diversity amongst the isolates of Xanthomonas axonopodis pv. citri causing Citrus canker in acid lime from different agro-climatic region of India ". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878),vol 1, no. 2, 2016, pp.302-315 AI Publications doi:10.22161/ijeab/1.2.25

Madhuri Katkar, K.S.Raghuwanshi, V. P. Chimote, S.G.Borkar, P.(2016).Pathological, Bio-chemical and Molecular diversity amongst the isolates of Xanthomonas axonopodis pv. citri causing Citrus canker in acid lime from different agro-climatic region of India . International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).1(2), 302-315.10.22161/ijeab/1.2.25

Madhuri Katkar, K.S.Raghuwanshi, V. P. Chimote, S.G.Borkar, P.(2016).Pathological, Bio-chemical and Molecular diversity amongst the isolates of Xanthomonas axonopodis pv. citri causing Citrus canker in acid lime from different agro-climatic region of India . International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).1(2), pp.302-315.

Madhuri Katkar, K.S.Raghuwanshi, V. P. Chimote, S.G.Borkar. 2016."Pathological, Bio-chemical and Molecular diversity amongst the isolates of Xanthomonas axonopodis pv. citri causing Citrus canker in acid lime from different agro-climatic region of India ". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).1(2):302-315.Doi:10.22161/ijeab/1.2.25

Madhuri Katkar, K.S.Raghuwanshi, V. P. Chimote, S.G.Borkar."Pathological, Bio-chemical and Molecular diversity amongst the isolates of Xanthomonas axonopodis pv. citri causing Citrus canker in acid lime from different agro-climatic region of India ", International Journal of Environment Agriculture and Biotechnology,vol.1,no. 2, pp.302-315,2016.

@article { madhurikatkar2016pathological,,
title={Pathological, Bio-chemical and Molecular diversity amongst the isolates of Xanthomonas axonopodis pv. citri causing Citrus canker in acid lime from different agro-climatic region of India },
author={Madhuri Katkar, K.S.Raghuwanshi, V. P. Chimote, S.G.Borkar , R},
journal={International Journal of Environment Agriculture and Biotechnology},
volume={1},
year= {2016} ,
}