Separation and Identification of Many Natural Products from Emblicaofficinalis Fruits and Study of their Antibacterial Activity

DOI: 10.22161/ijeab/3.6.33

Abstract:
The active constituents were separated from Emblicaofficinalis fruit, using column chromatography (CC) with solvent system of (pet.ether:ethyl acetate; 5:1) from petroleum ether extract (A1) and solvent system (chloroform:Methanol; 10:1) from Ethanol extract (A2). Saponification process was carried out on (A1F2) to separate many fatty acids and identification of five fatty acids (Heptanoic, Octanoic, Lauric, Palmitic and also cis& trans Oleic acids) using GLC-analysis. Moreover, acid hydrolysis was also carried out on (A2F2), to separate many free poles of phenolic compounds, such as (Hydroquinone and Gallic acid) were identified using HPLC-analysis. The active separated compounds under study showed different effects against the micro-organisms (m.o), using disc diffusion method turbidity.

Keywords:
GLC analysis, Emblicaofficinalis, fatty acid, phenolic compounds.

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