The Effect of Combination of Picloram and Bap on the Calculation of Clean Plants (Postogemon cablin Benth)

The Effect of Combination of Picloram and Bap on the Calculation of Clean Plants (Postogemon cablin Benth) ( Vol-4,Issue-1,January - February 2019 )

Author: Deasy Monica Latif, Warnita, Renni Mayerni

ijeab doi crossref DOI: 10.22161/ijeab/4.1.17

Keyword: Patchouli (Pegestemon cablin Benth), Picloram, BAP, Tissue Culture.

Abstract: This study aims to determine the best Picloram and BAP concentrations in InVitro patchouli callus formation. This experiment was conducted in November 2018 to January 2019 at the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University, Padang. This study used an experimental method with Completely Randomized Design (CRD) consisting of two factors. The first factor was the administration of Picloram concentration with 5 levels of treatment and the second factor was the administration of BAP concentrations with 5 levels of treatment. This experiment consisted of: This experiment consisted of Factor I: Picloram (A) Without Picloram (A1), Concentration of 1 mg / L (A2), Concentration of 2mg / L (A3), Concentration of 3 mg / L (A4), Concentration of 4mg / L (A5), Factor II: Concentration of BAP (S) Without BAP (S1), Concentration of 0.5 mg / L (S2), Concentration of 1 mg / L (S3), Concentration of 1.5 mg / L (S4) Concentration 2 mg / L (S5). The data obtained were analyzed using the F test at the level of 5%, if F count is greater than F table then the analysis is continued with the DMNRT test at the level of 5%. From the results of the study showed that the concentration of picloram had a significant effect on the growth of patchouli callus with an average callus appearance of 7 HST and percentage (%) of explants growing up to 100%. High picloram concentration did not significantly affect callus growth, 2 mg / l picloram + BAP concentration did not produce callus.

References:

[1] Abidin, Z. 1983. Basics of Knowledge About Growth Regulating Substances. Bandung: Angkasa.
[2] Aisyah, Y.2010. Characterization of Patchouli Oil (Pogostemon cablinBenth) and Increased Level of Patchouli Alcohold in Patchouli Oil Using Cellulose Acetate Membranes and Fractionation Distillation. Dissertation. Faculty of Agricultural Technology, Gadjah Mada University, Yogyakarta.
[3] Andaryani, S. 2010. Study of the Use of Various BAP and 2,4-D Concentrations in Induction of Jatropha CurcasL Callus (Jatropha Curcas L). Thesis. Faperta Sebelas Maret University. Surakarta
[4] Aprisa, R. 2012. Embryogenic Callus Induction of Two Corn Mutant Genotypes (Zea maysL.) In MS and N6 Basic Media. Essay. Department of Agronomy and Horticulture, Faculty of Agriculture, Agricultural Institute Bogor.
[5] Ardiana, D.W. 2009. The Technique of Giving Benzyl Amino Purin to Spur the Growth of Callus and Squash in Cotyledon Melon (Cucumis melo L.). Buletin Teknik Pertanian. 14(2): 5053
[6] Ariati, S.N. 2012. Induction of Cocoa Plant Callus (Theobroma cacao L.) On MS Media with Addition of 2,4-D, BAP and Coconut Water. Natural Science Journal. 1(1): 78-84
[7] Azizah, R. 2017. Growth of Liberika Coffee Callus Tungkal Jambi (Coffealiberica Var. Liberica Cv. Tungkal Jambi) with In Vitro Combination of 2,4-D and Kinetin. Thesis. Faculty of Agriculture, Jambi University.
[8] Chernova, L., K., Prokhorov M.N. and Filin Koldakov. 1975. Comparison of the dedifferentiating effects of 2,4-D and 4-amino-3,5,6-tricloropicolinic acid on tissue of legumes and cereals. Fiz. Rast. 22: 170-175.
[9] Darwati,I. 2007. Callus Culture and Hair Root Culture of Purwoceng (Pimpinellapruatjan Molk.) To Produce Secondary Metabolites. Dissertation. ITB Bogor Postgraduate School.
[10] Gardner, G.J.., R.B. Pearce and R.L. Mitchell. 1991. Cultivation Physiology (Translation of Herawati Susilo). UI. Press. Jakarta.
[11] George, E. F. 2008. Plant Propagation by Tissue Culture. Dordrecht: Springer.
[12] Gunawan, D, & Mulyani, S,. 2004. Natural Medicine (Farmakognosi) Jilid I. Jakarta: Penerbit Penebar Swadaya
[13] Gunawan, L. W. 1998. Network Culture Techniques. Bogor: PAU IPB.
[14] Habibah. 2009. Effectiveness of Addition of Jasmonic Acid Elistator in Increasing Synthesis of Andrographolide Bioactive Compounds in Sambiloto Cell Suspension Culture. Biosaintifika. 1(1): 11-18.
[15] Hendaryono, D.P.S. dan A. Wijayani 1994. Network Culture Techniques. Introduction and Guidelines for Vegetative Modern Propagation. Yogyakarta: Kanisius
[16] Indah, N., dan Ermavitalini, D. 2013 . Induction of Nyamplung Leaf Callus (Calophyllum inophyllum Linn). In Some Combinations of 6- Benzylaminopurine (BAP) and 2,4-Dichlorophenoxyatic Acid Concentrations (2,4 –D). Surabaya. Journal of science and art pomits. 2 (1): 2337 –3520.
[17] Kardinan. 2005. Fragrant Commodity Essential Oil Producing Plants Full of Potential. Jakarta: Penerbit Agromedia Pustaka.
[18] Karjadi dan Buchory. 2008. Effect of Basic Media Composition, Addition of BAP, and Pikloram Against Induction of Shallot Buds. J. Hort.18(1): 1-9
[19] Lestari E.G dan I Mariska, 2003. Effect of various media formulations on regeneration of indica rice callus. Proceedings of the Plant Pilot and Biotechnology Research Seminar, 257-263. Bogor, 23-24 September 2003.
[20] Lim, Z., Anna P., dan Sobri Hussein. 2009. Callus induction of Ocimum sanctumand estimation of its total flavonoid content. Asian Journal of Agricultural Sciences.1 (2):55-61.
[21] Litz, R.E and D.J. Gray. 1995. Somatic embryogenesis foragriculture improvment. World Jour.Microbiol. And Biotech. 11 : 416-425.
[22] Mahadi, I. 2008. Production of multiple shoots (Goniothalamus umbrosus J. Sinclair) by using the hormone kinetin and BAP in vitro. Agriculture Dynamics.23: 34-36.
[23] Mahadi, I., Wan Syafi’i dan Yeni Sari. 2016. Induction of Kasturi Orange Callus (Citrusmicrocarpa) Using 2,4-D and BAP with in vitro method. Journal of Indonesian Agricultural Sciences (JIPI). 21 (2): 84-89.
[24] Mangun, H. M. S. 2002. Patchouli. Jakarta: Penebar Swadaya.
[25] Nobert O, Zolta S, Be’la Da’nos. 2007. Influence of Different elicitorson the sunthesis of anthraquinone derivatives in Rubia tinctorum L. cell suspension cultures. Science Direct. Dyes and Pigments. 77: 249-257.
[26] Nugroho, Adi. 2008. Bussnies Plan. Jakarta: Universitas Indonesia. NuryaniY., Hobir, dan Syukur C., 2006. Patchouli Breeding Status (Pogostemon cablin Benth.). Technological development TRO.15 (2).
[27] Rukmana, R.2003. Patchouli Agribusiness Prospects and Cultivation Techniques. Penerbit Kanisius Yogyakarta.
[28] Santoso, H. B. 1991. Plant Patchouli. Yogyakarta: Kanisius.
[29] Santoso, U. dan Nursandi. 2004. Plant Tissue Culture. Malang: UMM Press.
[30] Tu, et.al.2001. Picloram.Weed Control Methods Handbook, The Nature Conservancy
[31] Zulkarnain. 2009. Plant Tissue Culture: Propagation Solution for Cultivation Plants. Jakarta: Bumi Aksara

Total View: 55 Downloads: 15 Page No: 105-110


Cite this Article:

MLA

Deasy Monica Latif, Warnita, Renni Mayerni et al."The Effect of Combination of Picloram and Bap on the Calculation of Clean Plants (Postogemon cablin Benth)". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878),vol 4, no. 1, 2019, pp.105-110 AI Publications doi:10.22161/ijeab/4.1.17

APA

Deasy Monica Latif, Warnita, Renni Mayerni, P.(2019).The Effect of Combination of Picloram and Bap on the Calculation of Clean Plants (Postogemon cablin Benth). International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).4(1), 105-110.10.22161/ijeab/4.1.17

Chicago

Deasy Monica Latif, Warnita, Renni Mayerni, P.(2019).The Effect of Combination of Picloram and Bap on the Calculation of Clean Plants (Postogemon cablin Benth). International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).4(1), pp.105-110.

Harvard

Deasy Monica Latif, Warnita, Renni Mayerni. 2019."The Effect of Combination of Picloram and Bap on the Calculation of Clean Plants (Postogemon cablin Benth)". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).4(1):105-110.Doi:10.22161/ijeab/4.1.17

IEEE

Deasy Monica Latif, Warnita, Renni Mayerni."The Effect of Combination of Picloram and Bap on the Calculation of Clean Plants (Postogemon cablin Benth)", International Journal of Environment Agriculture and Biotechnology,vol.4,no. 1, pp.105-110,2019.

Bibtex

@article { deasymonicalatif2019the,
title={The Effect of Combination of Picloram and Bap on the Calculation of Clean Plants (Postogemon cablin Benth)},
author={Deasy Monica Latif, Warnita, Renni Mayerni , R},
journal={International Journal of Environment Agriculture and Biotechnology},
volume={4},
year= {2019} ,
}