Extraction, chemical composition, use in induced protection and cross-reactive antigens between exopolisaccharides from Tremella fuciformis Berk and Xanthomonas campestris pv. citri (Hasse) Dye

DOI: 10.22161/ijeab/2.4.2

Keyword: Polysaccharides, fungi, bacterial, cross-reactive antigen.

Abstract: Exopolysaccharides (PS) are the major components on the surface of bacteria and also produced by fungi. These molecules are important in human health, in order to control diabetes as well as protect plants against attacks of foliage diseases. The objective of the present work was to study the partial chemical structure of the carbohydrate, use in control disease in plants and cross-serological relationship (cross-reactive antigens between isolates from fungi (Tremella fuciformis (Tf) and bacteria (Xanthomonas campestris pv. citri (Xcc)). Tf was developed in culture medium containing sorghum seeds during 20 days, and Xcc in the PDA (potato dextrose agar) medium for an 8 days period. The polysaccharide was removed from the culture medium, precipitated with ethanol, and quantified total sugar. By TLC was observed that 2 isolates presented galactose, glucose, mannose, arabinose and xylose in different proportions. Fucose and ribose was not found in the PS from Xcc but present in Tf. In ELISA, antiserum to Xcc revealed an antigenic homologous reaction with the same bacteria and heterologous with Tf. Barley plants pretreated with PS from Tf and later challenged with conidia from B.sorokiniana, demonstrated protection against the pathogen. Results suggested that PS from Tf presented induction of protection. Both PS (antigens) present an identical epitope demonstrated by reaction in Elisa test. The antibody against Xcc was specific for an epitope and bounded to another antigen due to having similar chemical properties.

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Erna Elisabeth Bach, Nilsa S.Y.Wadt,Vinicius O. Cardoso, Edgar Matias Bach Hi, Andresa Zamboni et al."Extraction, chemical composition, use in induced protection and cross-reactive antigens between exopolisaccharides from Tremella fuciformis Berk and Xanthomonas campestris pv. citri (Hasse) Dye". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878),vol 2, no. 4, 2017, pp.1452-1459 AI Publications doi:10.22161/ijeab/2.4.2

Erna Elisabeth Bach, Nilsa S.Y.Wadt,Vinicius O. Cardoso, Edgar Matias Bach Hi, Andresa Zamboni, P.(2017).Extraction, chemical composition, use in induced protection and cross-reactive antigens between exopolisaccharides from Tremella fuciformis Berk and Xanthomonas campestris pv. citri (Hasse) Dye. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).2(4), 1452-1459.10.22161/ijeab/2.4.2

Erna Elisabeth Bach, Nilsa S.Y.Wadt,Vinicius O. Cardoso, Edgar Matias Bach Hi, Andresa Zamboni, P.(2017).Extraction, chemical composition, use in induced protection and cross-reactive antigens between exopolisaccharides from Tremella fuciformis Berk and Xanthomonas campestris pv. citri (Hasse) Dye. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).2(4), pp.1452-1459.

Erna Elisabeth Bach, Nilsa S.Y.Wadt,Vinicius O. Cardoso, Edgar Matias Bach Hi, Andresa Zamboni. 2017."Extraction, chemical composition, use in induced protection and cross-reactive antigens between exopolisaccharides from Tremella fuciformis Berk and Xanthomonas campestris pv. citri (Hasse) Dye". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).2(4):1452-1459.Doi:10.22161/ijeab/2.4.2

Erna Elisabeth Bach, Nilsa S.Y.Wadt,Vinicius O. Cardoso, Edgar Matias Bach Hi, Andresa Zamboni."Extraction, chemical composition, use in induced protection and cross-reactive antigens between exopolisaccharides from Tremella fuciformis Berk and Xanthomonas campestris pv. citri (Hasse) Dye", International Journal of Environment Agriculture and Biotechnology,vol.2,no. 4, pp.1452-1459,2017.

@article { ernaelisabethbach2017extraction,,
title={Extraction, chemical composition, use in induced protection and cross-reactive antigens between exopolisaccharides from Tremella fuciformis Berk and Xanthomonas campestris pv. citri (Hasse) Dye},
author={Erna Elisabeth Bach, Nilsa S.Y.Wadt,Vinicius O. Cardoso, Edgar Matias Bach Hi, Andresa Zamboni , R},
journal={International Journal of Environment Agriculture and Biotechnology},
volume={2},
year= {2017} ,
}