Optimized recombinant Bacillus Subtilis 168 whole-cell catalyzes one-step biosynthesis of high fructose syrup

Author: Ildephonse Habimana, Qiao Zhina, Aqeel Sahibzada Muhammad, Jean Damascene Harindintwali, Abdulqader Al-Adeeb, Tolbert Osire, Waleed AL-Ansi, Mengkai Hu, Meijuan Xu, Xian Zhang, Zhiming Rao

ijeab doi crossref DOI: 10.22161/ijeab.62.6

Keyword: Glucose isomerase; isomerization; whole-cell catalysis; Bacillus Subtilis 168; high fructose syrup; biosynthesis.

Abstract: High fructose syrup is a sweetener that can replace sucrose, which is widely used in the food and beverage industries. In this study, codon-optimized Actinoplanes missouriensis CICIM B0118 (A) derived glucose isomerase heterologous expression was realized in the food-grade strain Bacillus Subtilis 168, and the recombinant Bacillus Subtilis 168/pMA5-xylA was successfully constructed. The whole-cell bioconversion system of D-glucose isomerization to the biosynthesis of D-fructose was optimized. The results showed that the concentration of biocatalysts was DWC 40 g/L, and the concentration of substrate D-glucose was 180 g/L, Mg2+ concentration 10 mmol/L, Co2+ concentration 1 mmol/L, continuous conversion at 70℃、220 R/min for 18 h, D-fructose concentration reached 103.32 g/L, the conversion rate was 57.4%, realizing the high fructose syrup one-step safe biosynthesis. This research provided an experimental and theoretical basis for the industrialized and safe biosynthesis of high fructose syrup and had an important reference value.

Article Info: Received: 21 Nov 2020; Received in revised form: 11 Feb 2021; Accepted: 01 Mar 2021; Available online: 24 Mar 2021

Total View: 1029 Downloads: 699 Page No: 067-075


Cite this Article:

MLA

Ildephonse Habimana, Qiao Zhina, Aqeel Sahibzada Muhammad, Jean Damascene Harindintwali, Abdulqader Al-Adeeb, Tolbert Osire, Waleed AL-Ansi, Mengkai Hu, Meijuan Xu, Xian Zhang, Zhiming Rao et al."Optimized recombinant Bacillus Subtilis 168 whole-cell catalyzes one-step biosynthesis of high fructose syrup". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878),vol 6, no. 2, 2021, pp.067-075 AI Publications doi:10.22161/ijeab.62.6

APA

Ildephonse Habimana, Qiao Zhina, Aqeel Sahibzada Muhammad, Jean Damascene Harindintwali, Abdulqader Al-Adeeb, Tolbert Osire, Waleed AL-Ansi, Mengkai Hu, Meijuan Xu, Xian Zhang, Zhiming Rao, P.(2021).Optimized recombinant Bacillus Subtilis 168 whole-cell catalyzes one-step biosynthesis of high fructose syrup. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).6(2), 067-075.10.22161/ijeab.62.6

Chicago

Ildephonse Habimana, Qiao Zhina, Aqeel Sahibzada Muhammad, Jean Damascene Harindintwali, Abdulqader Al-Adeeb, Tolbert Osire, Waleed AL-Ansi, Mengkai Hu, Meijuan Xu, Xian Zhang, Zhiming Rao, P.(2021).Optimized recombinant Bacillus Subtilis 168 whole-cell catalyzes one-step biosynthesis of high fructose syrup. International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).6(2), pp.067-075.

Harvard

Ildephonse Habimana, Qiao Zhina, Aqeel Sahibzada Muhammad, Jean Damascene Harindintwali, Abdulqader Al-Adeeb, Tolbert Osire, Waleed AL-Ansi, Mengkai Hu, Meijuan Xu, Xian Zhang, Zhiming Rao. 2021."Optimized recombinant Bacillus Subtilis 168 whole-cell catalyzes one-step biosynthesis of high fructose syrup". International Journal of Environment Agriculture and Biotechnology(ISSN: 2456-1878).6(2):067-075.Doi:10.22161/ijeab.62.6

IEEE

Ildephonse Habimana, Qiao Zhina, Aqeel Sahibzada Muhammad, Jean Damascene Harindintwali, Abdulqader Al-Adeeb, Tolbert Osire, Waleed AL-Ansi, Mengkai Hu, Meijuan Xu, Xian Zhang, Zhiming Rao."Optimized recombinant Bacillus Subtilis 168 whole-cell catalyzes one-step biosynthesis of high fructose syrup", International Journal of Environment Agriculture and Biotechnology,vol.6,no. 2, pp.067-075,2021.

Bibtex

@article { ildephonsehabimana2021optimized,
title={Optimized recombinant Bacillus Subtilis 168 whole-cell catalyzes one-step biosynthesis of high fructose syrup},
author={Ildephonse Habimana, Qiao Zhina, Aqeel Sahibzada Muhammad, Jean Damascene Harindintwali, Abdulqader Al-Adeeb, Tolbert Osire, Waleed AL-Ansi, Mengkai Hu, Meijuan Xu, Xian Zhang, Zhiming Rao , R},
journal={International Journal of Environment Agriculture and Biotechnology},
volume={6},
year= {2021} ,
}